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目的探讨大鼠脑缺血再灌氧化损伤及瓜蒌皮注射液的保护作用。方法 SD大鼠90只随机分为6组:假手术组、模型组、对照组、瓜蒌皮注射液低、中、高剂量组(分别给予不同剂量瓜蒌皮注射液),每组15只。假手术组和模型组给予生理盐水,对照组给予舒血宁注射液。各组腹腔注射给药,每组大鼠连续给药14d,末次给药1h后,采用线栓法制备MCAO模型。缺血2h后,再灌注24h。观察每组大鼠神经功能改变情况,测定每组大鼠脑组织中SOD活力、NO含量和MDA含量。结果瓜蒌皮注射液低、中、高剂量组的神经功能评分比模型组显著改善(P<0.05);瓜蒌皮注射液低、中、高剂量组的脑组织SOD活力比模型组显著升高(P<0.05);瓜蒌皮注射液低、中、高剂量组脑匀浆NO含量比模型组显著降低(P<0.05);瓜蒌皮注射液低、中、高剂量组脑匀浆MDA含量比模型组显著降低(P<0.05)。结论瓜蒌皮注射能够显著保护大鼠脑缺血再灌氧化损害,可能与提高SOD活力及降低MDA、NO有关。
Objective To investigate the protective effect of Guaopingpi Injection on cerebral ischemia-reperfusion injury in rats. Methods Ninety Sprague-Dawley rats were randomly divided into 6 groups: sham operation group, model group, control group, low, medium and high dose Guagua Pi injection group (given different doses of melon skin injection), 15 rats in each group . Rats in sham operation group and model group were given normal saline, and control group was given Shuxuening injection. Each group was administered by intraperitoneal injection. Each group of rats was given continuous administration for 14 days. After the last administration for 1 hour, MCAO model was established by thread plug method. After 2 hours of ischemia, reperfusion 24h. Changes of neurological function were observed in each group. SOD activity, NO content and MDA content in each group were determined. Results The scores of neurological function in the low, medium and high dose groups of Guandi Pi injection were significantly improved compared with the model group (P <0.05). The activity of SOD in brain of low, medium and high dose Gupepi injection group was significantly higher than that of the model group (P <0.05). The content of NO in brain homogenates of Gupepi injection group was significantly lower than that of model group (P <0.05), and that of Gupepi injection group was lower than that of model group MDA content was significantly lower than the model group (P <0.05). Conclusion Guandie injection can significantly protect against oxidative damage induced by cerebral ischemia-reperfusion in rats, which may be related to the increase of SOD activity and the decrease of MDA and NO.