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研究了浸种时间、诱导时间、6-BA浓度3个因素对吉育91胚尖不定芽诱导的影响,并将优化的胚尖再生体系与子叶节再生体系进行比较。结果表明,浸种24 h最适宜胚尖不定芽的形成,不定芽诱导率可达到85.1%,平均芽数可达到4.9个;诱导培养48 h时不定芽诱导率及平均芽数较高,分别为84.2%和5.3个;诱导培养基中6-BA浓度为2.0～3.0 mg.L-1时,不定芽诱导及伸长状况最佳。除了生根率差异不显著外,吉育91胚尖再生体系在再生能力与培养周期上均显著优于子叶节再生体系,因此更适合作为遗传转化的受体材料。
The effects of soaking time, induction time and 6-BA concentration on the induction of adventitious buds at embryo tip of Jiyu 91 were studied. The optimized embryogenic regeneration system was compared with that of cotyledonary node regeneration system. The results showed that the optimum adventitious bud formation was 24 h after soaking, the induction rate of adventitious buds reached 85.1% and the average number of buds reached 4.9. The induction rate and average shoot number of adventitious buds were higher at 48 h 84.2% and 5.3, respectively. When the concentration of 6-BA in induction medium was 2.0-3.0 mg.L-1, the induction and elongation of adventitious buds were the best. Except for the difference in rooting rate was not significant, the embryogenic shoot regeneration system of Jiyu91 was significantly better than that of cotyledonary node regeneration system in regeneration ability and culture cycle, so it was more suitable as a receptor material for genetic transformation.