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为研究树突状细胞(DC)融合人卵巢癌细胞体外诱导抗已知与未知肿瘤抗原的能力,从正常人和卵巢癌患者的外周血中分离单核细胞(MC),制备T细胞和DC;将酶分解后的原发灶与腹水的卵巢癌细胞(OVCA)与自体或同种异体DC融合制备抗原(OVCA/FC)后进行①流式细胞仪分析细胞表型;②T细胞扩增情况分析;③~(51)Cr释放法测定OVCA/FC刺激的T细胞细胞毒活性;④检测OVCA/FC刺激的细胞毒性T淋巴细胞的特异性。
To investigate the ability of dendritic cells (DCs) fused to human ovarian cancer cells to induce anti-known and unknown tumor antigens in vitro, monocytes (MCs) were isolated from the peripheral blood of normal and ovarian cancer patients and T cells and DCs were prepared (OVCA / FC) was obtained by fusing ovarian cancer cells (OVCA) with autologous or allogeneic DCs after primary enzymatic decomposition with ascites fluid; (1) Flow cytometry was used to analyze the cell phenotype; (2) T cell expansion The cytotoxic T lymphocytes stimulated by OVCA / FC were measured by ~ (51) Cr release assay. The specificity of OVCA / FC stimulated cytotoxic T lymphocytes was tested.