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5巯基胞嘧啶多核苷酸(5-Mercaptopolycytidylic acid,MPC)是DNA和RNA聚合酶的强有力抑制剂,亦能抑制白血病小鼠的骨髓和脾细胞的集落形成。德国临床试用表明MPC对小儿急性淋巴细胞性白血病有一定效果。本文报道MPC体外对人癌细胞株(肺癌A549,大肠癌HT-29,成骨肉瘤HOS)及正常羊膜细胞株(WISH)的抑制作用,并与Poly Ⅰ·MPC(系由等分子量的MPC和多次黄嘌呤核苷酸合成)作比较。为了了解这种抑癌作用究系MPC本身抑或由其降解产物所致,还同时观察了MPC、5巯基胞嘧啶核苷酸(SH~5-CMP)及5巯基胞嘧啶对上述细胞株的影响。
5-Mercaptopolycytidylic acid (MPC) is a potent inhibitor of DNA and RNA polymerase and also inhibits the colony formation of bone marrow and spleen cells in leukemic mice. German clinical trial showed that MPC has some effect on children with acute lymphoblastic leukemia. This article reports the inhibitory effect of MPC on human cancer cell lines (lung cancer A549, colorectal cancer HT-29, osteosarcoma HOS) and normal amniotic cell line (WISH) in vitro and in combination with Poly I MPC Multiple xanthine nucleotide synthesis) for comparison. In order to understand whether this tumor suppressor is caused by MPC itself or by its degradation products, the effects of MPC, 5-mercaptopyrimidine nucleotides (SH-5-CMP) and 5-mercaptocytosine on these cell lines were also observed .