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本文研究了铜 ( ) -新铜试剂 -谷胱甘肽 -乙醇体系的显色反应 ,探讨了反应条件。试验中发现乙醇可以使吸光度增加 13.8% ,提高了显色反应的灵敏度。该方法用于测定大豆提取液中谷胱甘肽的含量 ,取得了满意结果。
In this paper, the color reaction of copper () - new copper reagent - glutathione - ethanol system was studied and the reaction conditions were discussed. The test found that ethanol can increase the absorbance of 13.8%, increased the sensitivity of color reaction. The method was used to determine the content of glutathione in soybean extract, and satisfactory results were obtained.