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目的:观察Galectin-3及HPV16/18-E6蛋白在喉癌组织中的表达并分析其临床意义。方法:采用免疫组织化学法以及免疫印迹法，检测60例喉癌患者的癌组织及癌旁组织中Galectin-3蛋白和HPV16/18-E6蛋白的表达，并分析其与病例临床分期、病理分级等因素之间的关系。结果:免疫组织化学结果表明，Galectin-3蛋白表达的阳性率为78.3%(47/60)，免疫印迹的结果表明肿瘤组织中Galectin-3蛋白表达明显高于癌旁正常组织；统计学分析显示Glactin-3蛋白的表达与喉癌病例的临床分期、病理的分化程度以及淋巴结转移之间均具有相关性；与人乳头瘤病毒16/18-E6蛋白的表达无相关性。结论:Glactin-3蛋白的表达随着喉癌患者临床严重程度的加重呈逐渐增加的趋势，该指标的检测可为喉癌的早期诊断和治疗提供实验室参考。“,”Objective:To observe the expression and clinical significance of galectin-3 and HPV16/18-E6 proteins in laryngeal carcinoma patients.Methods:Immunohistochemistry and Western blot method were used to observe the expressions of galectin-3 protein and HPV16/18-E6 protein in laryngeal carcinoma tissues of 60 patients. n SAS 9.4 software was used to analyze the relationship between the galectin-3 protein and the clinical stage severity, pathological grades and lymph node metastasis in all the cases.n Results:The result of immunohistochemical examinations showed that the positive rate of galectin-3 protein expression was 78.3% (47/60), and the Western blot result showed that the galectin-3 protein expression in tumor tissues was significantly higher than that in adjacent normal tissues. Statistical analysis showed that galectin-3 expression was correlated with the severity of clinical staging, differentiation of the tumor and lymph node metastasis of laryngeal cancer. There was no correlation between the expression of galectin-3 protein and the 16/18-E6 protein of human papillomavirus.Conclusions:The expression of galectin-3 protein showed a gradually increasing trend with the aggravation of the clinical severity of laryngeal carcinoma, providing certain experimental data for the early diagnosis and treatment of laryngeal cancer.