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为了明确烟夜蛾Helicoverpa assulta G蛋白αq亚基(HassGαq)在雄性触角中的定位,进而探索该亚基在烟夜蛾嗅觉信号传导过程中的作用,本实验首先以原核表达的HassGαq蛋白作为抗原免疫Balb/c小鼠,制备了抗血清。SDS-PAGE分离雄性烟夜蛾触角匀浆上清液后,Western blot检测结果表明,所制备的抗血清可识别HassGαq蛋白,同时也与非目的蛋白发生反应,说明在雄性烟夜蛾触角中HassGαq基因可能有多个转录本,或者可能有多种G蛋白α亚基表达。此外,商品化的anti-Gq/11α抗血清可特异地识别HassGαq蛋白。因此,利用anti-Gq/11α抗血清和免疫组化方法对HassGαq蛋白在雄性烟夜蛾触角中进行定位,结果显示HassGαq在雄性烟夜蛾触角毛形感器和锥形感器的基部以及感器腔中皆有表达。据此推测HassGαq可能参与烟夜蛾的嗅觉信号传导。
In order to elucidate the localization of αq subunit α (HassGαq) of Helicoverpa assulta G protein in male antennae and to explore the role of this subunit in the olfactory signal transduction of H. nothoides, HassGαq prokaryotic expression was used as antigen Balb / c mice were immunized and antisera were prepared. Western blot results showed that the prepared antiserum can recognize HassGαq protein and also react with non-target protein, indicating that the HassGαq Genes may have multiple transcripts or may have multiple G protein alpha subunits. In addition, the commercial anti-Gq / 11α antiserum specifically recognizes the HassGαq protein. Therefore, anti-Gq / 11α antiserum and immunohistochemistry were used to locate HassGαq protein in the antennae of H. malayanus. The results showed that HassGαq was located at the base of sensilla and sensilla, Cavities are expressed. Therefore, it is speculated that HassGαq may be involved in the olfactory signal transduction of H. armigera.