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以聚乙二醇(PEG)为还原剂、溶剂和修饰剂,将乙酰丙酮铁(Fe(acac)_3)高温热分解合成超顺磁性氧化铁纳米粒子(Superparamagnetic iron oxide nanoparticles,SPIONs)。透射电镜(TEM)结果显示:纳米粒子形状规则,分布均匀,平均粒径为7.5±1.0 nm。XRD结果表明样品主相为结晶良好的Fe_30_4。通过将SPIONs表面修饰的PEG与马来酸酐(Mal)结合,再借助EDC-NHS的方法,分别与多肽(angiopep-2,ANG)或转铁蛋白(transferrin,Tf)接枝。结果表明:修饰ANG的SPIONs的水合动力学粒径为42 nm,zeta电位为-9.9 mV,ANG的修饰量为19 wt%,饱和磁化强度为58emu/g;修饰Tf的SPIONs的水合动力学粒径为96 nm,zeta电位为2.3 mV,Tf的修饰量为10 wt%,饱和磁化强度为43 emu/g。红外分析表明ANG或Tf分别共同修饰在SPIONs表面。修饰物使纳米粒子具有良好了水分散性。本工作为SPIONs应用于生物医学研究建立了材料基础。
The Fe (acac) _3 was thermally decomposed into superparamagnetic iron oxide nanoparticles (SPIONs) by using polyethylene glycol (PEG) as reducing agent, solvent and modifier. Transmission electron microscopy (TEM) results showed that the nanoparticles had regular shape and uniform distribution with average diameter of 7.5 ± 1.0 nm. XRD results show that the main phase of the sample is well-crystallized Fe_30_4. The peptides (angiopep-2, ANG) or transferrin (Tf) were grafted by coupling SPIONs surface-modified PEG to maleic anhydride (Mal) and then to EDC-NHS. The results showed that the hydration kinetic particle size of SPIONs modified with ANG was 42 nm, the zeta potential was -9.9 mV, the modification amount of ANG was 19 wt% and the saturation magnetization was 58 emu / g. Hydration kinetics of SPIONs modified Tf The diameter was 96 nm, the zeta potential was 2.3 mV, the modification amount of Tf was 10 wt%, and the saturation magnetization was 43 emu / g. Infrared analysis showed that ANG or Tf co-modified surface of SPIONs respectively. Modifiers give the nanoparticles good water dispersibility. This work establishes the material foundation for the application of SPIONs to biomedical research.