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美国弗吉尼亚大学Mishin等用基因重组技术删去甲型流感病毒〔A/Bayern/7/95类似株(H1N1)〕神经氨酸酶(NA)基因而构建了无NA的变异株(delNA-mutant),以及用一种编码嵌合蛋白(eGFP,由绿色荧光蛋白胞质尾区、跨膜区和NA主要区融合而成)基因插入取代NA基因而构建了另一变异株(delNA
Mnain at the University of Virginia et al. Constructed a NAA-free mutant by deleting the NA gene [A / Bayern / 7/95 (H1N1)] neuraminidase gene by gene recombination technology. , As well as inserting a substitutional NA gene with a gene encoding a chimeric protein (eGFP fused to the green fluorescent protein cytoplasmic tail, transmembrane region and NA major region) to construct another variant