Effect of antiallergic herbal agents on chloride channel-3 and immune microenvironment in nasal muco

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Background Allergic rhinitis (AR) is a Th2 dominant cytokine response. Chloride channel-3 (CIC-3) plays an important role in nasal mucosal edema and inflammatory pathologic changes in AR. Antiallergic herbal agents (AHA) are antiallergic herbal products. In the previous study, we have demonstrated that AHA clearly inhibited allergic medium and relieved allergic reaction of AR. The aim of this study was to evaluate the function of CIC-3 and discuss the possible therapeutic effects of AHA on immune microenvironment in AR.Methods AHA were produced and used to treat AR. An animal model of an AR rabbit was established by ovalbumin (OVA). The rhinitis rabbits were randomly divided into three groups: AHA treated group (AHATG), model group (MG) and healthy control group (HCG). The expressions of CIC-3 protein were examined by immunohistochemical method. The mucosal epithelial cells of all the rabbit groups were primarily cultured with tissue culture method in vitro with or without rhlL-4 or rhlL-2. Furthermore, the expressions of CIC-3 mRNA were detected by real-time PCR. The levels of monocyte chemotactic factor-1 (MCP-1) and vascular cell adhesion molecule-1 (VCAM-1) protein in culture supatants were measured by ELISA.Results The expressions of CIC-3 mRNA increased more in mucosal epithelial cells of MG than those in AHATG and HCG (P<0.01). The levels of CIC-3 mRNA, MCP-1 and VCAM-1 protein in culture supatants of MG were significantly higher than those in the other two groups (P<0.01). Those were significantly increased in MG untreated 12 hours later than those in other two groups (P <0.01). The expressions of CIC-3 mRNA, MCP-1 and VCAM-1 protein in culture supatants of MG and HCG treated with rhlL-4 were significantly higher than those in the AHATG treated with rhlL-4 (P <0.01). The levels of CIC-3 mRNA, MCP-1 and VCAM-1 protein in culture supatants of all groups treated with rhlL-2 showed no significant changes (P>0.05).Conclusions AHA can inhibit the secretions of CIC-3, MCP-1 and VCAM-1 in mucosal epithelia and improve inflammatory reaction of AR. CIC-3 plays an important role in the secretion of cytokines and mucosal inflammatory response in AR. RhlL-4 can enhance the secretion of CIC-3, MCP-1 and VCAM-1 in mucosal epithelial cells, especially during the AR process. These enhanced effects of rhlL-4 were significantly suppressed by AHA. The secretions of CIC-3, MCP-1 and VCAM-1 can not be induced obviously by rhlL-2 in mucosal epithelial cells in AR.
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