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建立了大鼠服用厚朴提取物后的血清中及尿中厚朴酚与和厚朴酚的高效液相色谱测定法。色谱柱填料为Spherisorb C1 8,流动相为甲醇 -水 -冰醋酸 (体积比为 70∶ 30∶ 1) ,U V检测波长为 2 94 nm,灵敏度 0 .0 0 5AU FS。样品用甲醇沉淀蛋白 ,上清液酸化后用乙酸乙酯 -乙醚萃取 ,然后测定其中的药物浓度。血清和尿中的药物浓度与峰面积的线性关系良好 ,线性范围分别为 0 .0 5～ 2 mg/L(厚朴酚 )、0 .0 2 5～ 1mg/L (和厚朴酚 ) ;精密度和重现性良好。血清中厚朴酚与和厚朴酚的平均加样回收率分别为 95.6% ( RSD=3.85% )和 93.8% ( RSD=3.95% ) ,尿中分别为 96.0 % ( RSD=3.83% )和 94 .9% ( RSD=3.54 % )。药物在血清中和尿中的最低检测限分别为 0 .0 2和 0 .0 4 mg/L。该法适于血清和尿中厚朴酚与和厚朴酚的药物浓度监测
A HPLC method was established for determination of honokiol and honokiol in serum and urolithin after taking Magnolia biondii extract in rats. The column packing was Spherisorb C1 8 with the mobile phase of methanol-water-glacial acetic acid (volume ratio of 70:30:1). The UV detection wavelength was 2 94 nm and the sensitivity was 0. 05 5A FS. Samples were precipitated with methanol, the supernatant acidified with ethyl acetate - ether extraction, and then determine the drug concentration. The linear relationship between drug concentration and peak area in serum and urine was good. The linear range was 0. 05 ~ 2 mg / L (honokiol), 0. 02 ~ 1 mg / L Good precision and reproducibility. The mean recoveries of serum honokiol and honokiol were 95.6% (RSD = 3.85%) and 93.8% (RSD = 3.95%), respectively, and 96.0% (RSD = 3.83%) and 94 .9% (RSD = 3.54%). The lowest limit of detection of the drug in serum and urine was 0. 02 and 0. 0 4 mg / L respectively. The method is suitable for monitoring serum and urinary honokiol and honokiol drug concentration