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目的 研究银杏叶提取物 (extractsofGinkgobiloba ,EGb76 1)、肌酸和氨哮素延缓去神经骨骼肌萎缩的效果及其机制。 方法 复制大鼠臂丛神经损伤动物模型 ,2 4只SD大鼠随机分为EGb76 1组、肌酸组、氨哮素组和对照组 ,分别以相应药物 ( 10 0mg·kg 1·d 1)及等渗盐水灌胃 ,5周后检测各组臂围、肌湿重、肌肉总蛋白含量 ,用末端转移酶介导的dUTP缺口末端标记法 (TdT -me diateddUTPnickendlabeling,TUNEL)检测萎缩肌肉中的细胞凋亡 ,用免疫组织化学染色及灰度分析检测肌细胞中fas、FLICE/Caspase8表达的变化。 结果 EGb76 1、肌酸和氨哮素不同程度地抑制去神经骨骼肌萎缩大鼠的臂围、肌肉湿重和肌肉总蛋白含量的降低 (P <0 .0 5 ) ,减少萎缩肌肉中的TUNEL染色阳性细胞数以及细胞中的fas、FLICE/Caspase8含量 (P <0 .0 1)。 结论 EGb76 1、肌酸和氨哮素能够有效地延缓去神经骨骼肌萎缩 ,其机制可能与抑制肌细胞凋亡有关。
Objective To investigate the effects of extracts of Ginkgo biloba (EGb76), creatine and aminin on the atrophy of denervated skeletal muscles and their mechanisms. Methods Animal models of brachial plexus injury were duplicated in rats. 24 SD rats were randomly divided into EGb76 group 1, creatine group, clenbuterol group and control group, with corresponding drugs (10 0 mg·kg 1·d 1). Iso-osmotic saline was intragastrically administered. After 5 weeks, the arm circumference, muscle wet weight, and total muscle protein content were measured. TdT-mediatedd UTP nickend labeling (TUNEL) was used to detect atrophic muscles. Apoptosis was detected by immunohistochemical staining and gray-scale analysis to detect the changes of fas and FLICE/Caspase8 expression in myocytes. RESULTS: EGb76 1. Creatine and amidin inhibited the decrease of arm circumference, muscle wet weight and total muscle protein content in rats with atrophic skeletal muscle atrophy (P < 0.05), and reduced TUNEL in atrophic muscles. The number of positive cells stained and the content of fas and FLICE/Caspase8 in the cells (P < 0.01). Conclusion EGb76 1, creatine and aminomycin can effectively delay atrophy of denervated skeletal muscle, and its mechanism may be related to the inhibition of apoptosis of myocytes.