论文部分内容阅读
目的:探讨99m锝-甲氧基异丁基异腈(technetium-99m methoxyisobutylisonitrile,99mTc-MIBI)在人肺腺癌细胞株A549及其紫杉醇耐药株A549/taxol中的摄取与肺癌耐药之间的关系,以及二氯乙酸盐(dichloroacetate,DCA)对A549和A549/taxol细胞耐药的影响。方法:用CCK-8法检测不同浓度DCA对A549/taxol细胞生长的影响以及不同浓度紫杉醇单独或联合DCA(10mmol/L)对A549和A549/taxol细胞的半数抑制浓度(half inhibitory concentration,IC50)值。γ计数器检测DCA(10mmol/L)作用后A549和A549/taxol细胞对99mTc-MIBI的清除情况。结果:DCA≤10mmol/L时,对A549/taxol细胞无明显毒性作用。紫杉醇对A549/taxol细胞的IC50值明显高于对A549细胞(P<0.01);与紫杉醇单独作用组比较,A549/taxol细胞紫杉醇联合DCA组的IC50值下降(P<0.01)。A549/taxol细胞对99mTc-MIBI的清除率高于A549细胞(P<0.01),DCA处理后可使A549/taxol细胞对99mTc-MIBI的清除率下降(P<0.05);DCA并不影响A549细胞对99mTc-MIBI的清除率。结论:低毒浓度DCA可有效逆转A549/taxol细胞对紫杉醇的耐药,99mTc-MIBI的清除率可用于检测A549/taxol细胞的耐1药情况。
Objective: To investigate the relationship between uptake of 99m technetium-99m methoxyisobutylisonitrile (99mTc-MIBI) and human lung adenocarcinoma cell line A549 and its paclitaxel resistant A549 / taxol and lung cancer drug resistance , And dichloroacetate (DCA) on A549 and A549 / taxol cell resistance. Methods: The effects of different concentrations of DCA on the growth of A549 / taxol cells and the half inhibitory concentration (IC50) of paclitaxel alone or in combination with DCA (10 mmol / L) on A549 and A549 / taxol cells were detected by CCK- value. γ counter was used to detect the clearance of 99mTc-MIBI in A549 and A549 / taxol cells by DCA (10 mmol / L). Results: When DCA≤10mmol / L, there was no obvious toxic effect on A549 / taxol cells. The IC50 value of paclitaxel in A549 / taxol cells was significantly higher than that in A549 cells (P <0.01). Compared with paclitaxel alone group, the IC50 of paclitaxel and DCA group decreased in A549 / taxol cells (P <0.01). The clearance rate of 99mTc-MIBI in A549 / taxol cells was higher than that of A549 cells (P <0.01). The clearance rate of 99mTc-MIBI in A549 / taxol cells was decreased after DCA treatment (P <0.05) Clearance rate of 99mTc-MIBI. CONCLUSION: Low dose DCA can effectively reverse the drug resistance of paclitaxel in A549 / taxol cells. The clearance rate of 99mTc-MIBI can be used to detect the drug resistance of A549 / taxol cells.