卵磷脂—胆固醇酰基转移酶(以下简称LCAT)催化下列反应:把卵磷脂C_2位上的脂肪酸转移到非酯化胆固醇上,生成溶血卵磷脂和胆固醇脂。反应在血浆内进行。人体胆固醇代谢运转中,该酶是关键环节之一,故测定LCAT活性对研究冠心病的脂质代谢实属十分必要。LCAT的活性测定方法大体上有三类:①免疫学方法①,“②共同基质法”(the common—substrate method),③自身基质法 Lecithin - cholesterol acyltransferase (hereinafter referred to as LCAT) catalyzed the following reactions: the lecithin C 2 position fatty acid transfer to non-esterified cholesterol, the formation of lysolecithin and cholesterol. The reaction is carried out in plasma. In the process of human cholesterol metabolism, the enzyme is one of the key links. Therefore, it is very necessary to determine the activity of LCAT to study the lipid metabolism of coronary heart disease. There are generally three types of LCAT assay methods: ① immunological methods ①, “the common-substrate method”, ③ self-matrix method