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目的从分子水平检测急性重症呼吸综合征(SARS)患者的病变组织中SARS病毒(SARS-associated coronavirus, SARS-CoV)的存在和分布情况。方法应用原位杂交技术检测因SARS死亡患者的肺、脾脏、淋巴结、垂体、胰腺、甲状旁腺、肾上腺、胃肠道、皮肤、脑、肝、肾、血管、四肢横纹肌组织、骨髓、心脏、卵巢、子宫和睾丸等组织的SARS-CoV RNA的表达和定位。结果尸检组织多部位(包括肺泡上皮细胞、气管及支气管浆液腺上皮细胞、肺内单核/巨噬细胞、脾脏和淋巴结的单核/巨噬细胞、胰腺腺泡细胞、垂体嗜酸性细胞、肾上腺皮质细胞、甲状旁腺嗜酸性细胞、食道鳞状上皮、胃肠道上皮细胞及胃粘膜壁细胞、皮肤汗腺细胞、大脑神经元细胞、肝细胞、肾远曲小管上皮细胞、骨髓早幼粒细胞及小静脉内皮细胞)SARS-CoV RNA阳性。结论SARS-CoV可侵犯全身多种器官;SARS-CoV在机体的分布情况与冠状病毒受体CD13分布相似;皮肤汗腺、消化道上皮及肾远曲小管上皮细胞SARS-CoV RNA阳性对确定SARS传播途径具有重要意义。
Objective To detect the presence and distribution of SARS-associated coronavirus (SARS-CoV) in the diseased tissues of patients with acute severe respiratory syndrome (SARS) at the molecular level. Methods In situ hybridization was used to detect striated muscle, bone marrow, heart, lung, spleen, lung, spleen, lymph nodes, pituitary, pancreas, parathyroid, adrenal gland, The expression and localization of SARS-CoV RNA in tissues such as ovary, uterus and testis. RESULTS: The autopsy tissues had multiple sites (including alveolar epithelial cells, trachea and bronchial serous glandular epithelial cells, intrapulmonary monocytes / macrophages, monocytes / macrophages in spleen and lymph nodes, pancreatic acinar cells, pituitary eosinophils, Cortical cells, parathyroid eosinophils, esophageal squamous epithelium, gastrointestinal epithelial cells and gastric mucosa parietal cells, skin sweat gland cells, brain neurons, hepatocytes, renal far tubule epithelial cells, bone marrow promyelocytic And venule endothelial cells) SARS-CoV RNA positive. Conclusion SARS-CoV can invade whole body and multiple organs. The distribution of SARS-CoV in the body is similar to the distribution of coronavirus receptor CD13. SARS-CoV RNA positive in dermal sweat gland, digestive epithelium and renal distal tubule epithelial cells. Way is of great significance.