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以棉铃虫为对象，采取喷雾和喂食两种接种方法，研究了冰核细菌对昆虫的结冰温度的影响。结果表明：两种方法接种的冰核细菌均能明显提高棉铃虫的结冰温度，但不同接种方法间存在差异。喷雾接种的虫体结冰温度比对照高５．１４－１０．７９℃，接种１天后就将结冰温度提高５．９０℃，在棉铃虫化蛹后还能将结冰温度提高７．７１℃。喂食接种虫体接种两天后才与对照的结冰温度之间出现明显的差异，能将结冰温度提高２．２５－４．５６℃，化蛹后仅能将结冰温度提高０．９３℃。试验证明，冰核细菌能在昆虫体上起到异源冰核的作用，从而提高虫体的结冰温度。为探索一条利用生物冰核降低越冬害虫的过冷却能力，诱发虫体在较高温度下发生结冰，破坏细胞组织，造成虫体死亡，以此来提高越冬害虫的死亡率，压低来年虫源，减轻危害的促冻杀虫新途径提供了科学依据。
Taking Helicoverpa armigera as object, two methods of inoculation and feeding were used to study the effect of ice nucleation bacteria on icing temperature of insects. The results showed that both methods could significantly increase the freezing temperature of cotton bollworm, but there were differences among different inoculation methods. The temperature of icing inoculation was 5.14-10.79 ℃ higher than that of the control, and the icing temperature increased 5.90 ℃ after 1 day of inoculation, and the icing temperature was 7.71 ℃. Two days after inoculation, there was a significant difference between the freezing temperature and the control, which could increase the freezing temperature by 2.25-4.56 ℃. After the pupa, only the freezing temperature increased by 0.93 ℃ . Experiments show that ice nucleating bacteria can play the role of heterologous ice nucleus in insects, thereby increasing the body temperature of freezing. In order to explore a use of biological ice nuclei to reduce over-cooling overwintering pests ability to induce the body to freeze at higher temperatures, destruction of cell tissue, resulting in death of parasites, in order to improve the mortality of overwintering pests, , To reduce the harm of the new means of freezing and thawing insecticide provides a scientific basis.