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用同步辐射微束X射线荧光(μ-SRXRF)技术结合激光扫描共聚焦显微成像(LSCM)技术研究了经CdSe@ZnS量子点口暴露的果蝇成虫及其子代一期幼虫体内的微区分布。μ-SRXRF分析表明,果蝇成虫摄取CdSe@ZnS量子点后,果蝇成虫胃肠道组织中有Cd、Zn和Se元素的富集,说明量子点经果蝇口服暴露后主要富集在肠道和生殖道部位。LSCM观察表明,CdSe@ZnS量子点暴露后成虫所产一期幼虫的生殖部位(精巢)有明显的荧光增强信号,表明量子点暴露能够经果蝇母体转运到子代。μ-SRXRF分析技术为纳米颗粒在活体内转运研究提供了很好的分析手段。
The effects of micro-beam X-ray fluorescence (μ-SRXRF) and laser scanning confocal microscopy (LSCM) on the first-instar larvae of Drosophila exposed to CdSe @ ZnS QDs were studied. District distribution. The results of μ-SRXRF showed that Cd, Zn and Se were enriched in the gastrointestinal tissues of Drosophila adults after the Drosophila adults ingested CdSe @ ZnS quantum dots, indicating that the QDs were mainly enriched in the intestines after oral exposure to Drosophila melanogaster Road and genital tract site. The results of LSCM showed that there was a significant fluorescence enhancement signal in the reproductive part (testis) of the first larvae of adult worms exposed to CdSe @ ZnS quantum dots, indicating that the quantum dot exposure can be transferred to the offspring through the Drosophila mother. The μ-SRXRF analysis technique provides a good analytical tool for the study of nanoparticle transport in vivo.