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TRAIL(TNF relatedapoptosis inducingligand ,简称TRAIL或Apo 2L)是肿瘤坏死因子家族的新成员 ,体外研究表明其具有特异性杀伤肿瘤细胞的功能 .为了进一步探索TRAIL的生物学功能及其临床应用前景 ,首次从中国正常人外周血淋巴细胞中扩增了TRAIL胞外功能区(sTRAIL) ,并将其插入原核表达载体 ,转化大肠杆菌后 ,可溶型重组蛋白 (rsTRAIL)获得了高效稳定表达 ,其产量达到菌体总蛋白的 30 %左右 .体外研究表明 ,亲和层析纯化后的重组蛋白在 0 .1～ 1 μg/mL的浓度范围内 ,能显著诱导所试全部 7种肿瘤细胞株细胞凋亡 ,但不能诱导正常鼠成纤维细胞株细胞凋亡 ;进一步研究表明 ,rsTRAIL对贲门癌、乳腺癌和恶性胸腺瘤等原代细胞也具有强烈的杀伤作用 ,但不能杀伤正常人外周血淋巴细胞 .此实验结果为rsTRAIL特异性地诱导肿瘤细胞凋亡的生物学作用提供了新的实验证据 ,具有重要的临床应用价值 .
TRAIL (TNF relatedapoptosis inducingligand, referred to as TRAIL or Apo 2L) is a new member of the tumor necrosis factor family, in vitro studies have shown that it has specific anti-tumor cell function.In order to further explore the biological function of TRAIL and its clinical application prospects, for the first time from The TRAIL extracellular domain (sTRAIL) was amplified from normal human peripheral blood lymphocytes and inserted into the prokaryotic expression vector. After transformation into E. coli, the soluble recombinant protein (rsTRAIL) was efficiently and stably expressed, and its yield reached 30% of the total bacterial protein in vitro studies have shown that affinity chromatography purified recombinant protein in the range of 0.1-1 μg / mL, can significantly induce the apoptosis of all seven tumor cell lines tested , But could not induce normal murine fibroblast cell apoptosis; further studies have shown that rsTRAIL on cardiac cancer, breast cancer and malignant thymoma and other primary cells also have a strong killing effect, but can not kill normal peripheral blood lymphocytes. This experimental result provides new experimental evidence for the biological role of rsTRAIL in specifically inducing tumor cell apoptosis and has important clinical With value.