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目的建立人巨细胞病毒(HCMV)感染Balb/c小鼠模型,探索HCMV感染椎间盘的依据。方法取不同剂量组HCMV AD169株腹腔注射6~8周SPF级Balb/c小鼠♀♂各6只,同时设立灭活病毒对照组6只和细胞对照组6只。取各组小鼠椎间盘组织进行病毒分离与鉴定;采用PCR和RT-PCR分别检测HCMV UL83基因和UL54基因及其相应基因的转录;取椎间盘组织行HE染色病理并观察。结果2×106和2×105PFU/m l病毒组♀小鼠病毒分离、PCR及RT-PCR均为阳性;HE染色可见椎间盘组织局部灶性淋巴细胞浸润等慢性炎症反应,纤维环板层结构紊乱,关节软骨钙化层增厚,大量黏液及泡沫细胞,软骨下血管明显减少。♂小鼠、其它剂量病毒组、灭活病毒组和HF对照组均为阴性。结论HCMV可以感染小鼠椎间盘组织,并与感染病毒剂量有关;人巨细胞病毒感染与椎间盘变性有相关性,可能是椎间盘退变的一个重要危险因素。
Objective To establish a model of Balb / c mouse infected with human cytomegalovirus (HCMV) and explore the basis of HCMV infection in the intervertebral disc. Methods Different doses of HCMV AD169 strain were injected intraperitoneally with 6 mice of 6 weeks to 8 weeks of SPF grade Balb / c mice, and 6 mice in control group and 6 mice in control group were established. The intervertebral disc tissue of each group was isolated and identified by virus isolation. The transcription of HCMV UL83 gene and UL54 gene and their corresponding genes were detected by PCR and RT-PCR, respectively. The intervertebral disc tissue was examined by HE staining. Results The virus was isolated from 2 × 106 and 2 × 105 PFU / ml mice, and PCR and RT-PCR were positive. HE staining showed chronic inflammatory reaction such as focal focal lymphocyte infiltration of disc tissue, Articular cartilage calcification thickening, a large number of mucus and foam cells, subchondral vessels was significantly reduced. ♂ mice, other doses of virus group, inactivated virus group and HF control group were negative. Conclusion HCMV can infect the intervertebral disc tissue of mice and is related to the dose of virus infection. Human cytomegalovirus infection is associated with disc degeneration, which may be an important risk factor for disc degeneration.