目的 检测miR-373在骨肉瘤细胞中表达情况,并探讨其对细胞增殖、侵袭和迁移的影响.方法 体外培养人骨肉瘤细胞株SJSA-1及人骨肉瘤细胞hFOB 1.19,将SJSA-1随机分为4组,采用FuGENE(R) HD转染试剂转染细胞,4组细胞分别为空白对照组(CK组)、阴性对照组(NC组)、miR-373过表达质粒组(miR-373 mimic组)及miR-373抑制质粒组(miR-373 inhibitor组),转染48 h后,qRT-PCR法检测miR-373的相对表达量,CCK-8法检测细胞增殖情况,Transwell法检测细胞侵袭与迁移的情况.结果 SJSA-1细胞miR-373相对表达量明显高于hFOB 1.19细胞(P＜0.05);与CK组、NC组比较,miR-373 mimic组细胞miR-373相对表达量明显升高(P＜0.05),miR-373 inhib-itor组细胞miR-373相对表达量明显降低(P＜0.05);miR-373 mimic组培养24、36、48 h的OD值均明显高于CK组、NC组(P＜0.05),miR-373 inhibitor组培养24、36、48 h的OD值均明显低于CK组、NC组(P＜0.05);miR-373 mimic组细胞迁移数目明显多于CK组、NC组(P＜0.05),miR-373 inhibi-tor组细胞迁移数目明显少于CK组、NC组(P＜0.05);miR-373 mimic组细胞侵袭数目明显多于CK组、NC组(P＜0.05),miR-373 inhibitor组细胞侵袭数目明显少于CK组、NC组(P＜0.05).结论 骨肉瘤细胞中miR-373表达上调,miR-373过表达可促进骨肉瘤细胞的增殖、侵袭和迁移,miR-373低表达可抑制骨肉瘤细胞的增殖、侵袭和迁移,有望成为临床骨肉瘤诊断和治疗的重要靶点.“,”Objective To detect the expression of miR-373 in osteosarcoma cells and explore its effects on cell proliferation,invasion,and migration.Methods Human osteosarcoma cell line SJSA-1 and human osteoblast hFOB 1.19 cultured in vitro.Human osteosarcoma cell line SJSA-1 were randomly divided into four groups:blank control group (CK group),negative control group (NC group),miR-373 over-expressed plasmid group (miR-373 mimic group) and miR-373 inhibited plasmid group (miR-373 inhibitor group).Cells were transfected with FuGENE(R) HD transfection reagent.After 48 hours of transfection,the relative expression of miR-373 was detected by quantitative real-time polymerase chain reaction (qRT-PCR),cell proliferation by cell counting kit-8 (CCK-8) and cell invasion and migration by Transwell.Results The relative expression of miR-373 in SJSA-1 cells was significantly higher than that in hFOB-1.19 cells (P ＜ 0.05);compared with CK group and NC group,the relative expression of miR-373 in the miR-373 mimic group increased significantly (P ＜ 0.05),and the relative expression of miR-373 in the miR-373 inhibitor group was significantly lower than that in the control group (P ＜ 0.05);optical density (OD) value of miR-373 mimic group at 24 h,36 h,and 48 h were significantly higher than that of CK group and NC group (P ＜ 0.05),and OD value of miR-373 inhibitor group at 24 h,36 h,and 48 h were significantly lower than that of CK group and NC group (P ＜ 0.05);the number of cell migration in the miR-373 mimic group was significantly higher than that in the CK group and NC group (P ＜ 0.05),and the number of cell migration in the miR-373 inhibitor group was significantly lower than that in CK group and NC group (P ＜ 0.05);the number of cell migration in the miR-373 mimic group was significantly higher than that in the CK group and NC group (P ＜ 0.05),and the number of cell migration in the miR-373 inhibitor group was significantly lower than that in CK group and NC group (P ＜ 0.05).Conclusions The expression of miR-373 is up-regulated in osteosarcoma.Overexpression of miR-373 can promote the proliferation,invasion,and migration of osteosarcoma cells,while the low expression of miR-373 can inhibit the proliferation,invasion,and migration of osteosarcoma cells,which may be an important target for the diagnosis and treatment of osteosarcoma.