目的:应用串联质谱技术对惠州博罗地区围绝经期女性进行维生素D水平检测,探讨其与骨密度的相关性。方法:随机选择2013年1月至2016年1月间于博罗县人民医院就诊年龄46~54岁围绝经期女性530例作为研究对象,进一步根据维生素D的组成进行分析,观察各组差异主要存在于维生素D_2还是D_3,最后使用双能X线检测各组骨密度,分析其与维生素D水平是否存在相关性。结果:结果显示4组维生素D_2水平组内及组间差异均无统计学意义(P>0.05),主要差异存在维生素D_3水平的组内及组间,差异具有统计学意义(P<0.05)。4组腰椎及全髋骨密度随维生素D水平升高呈递增趋势,差异具有统计学意义(P<0.05)。结论 :基于串联质谱技术的惠州博罗地区围绝经期女性血清维生素D水平,大部分处于分充足状态,维生素D缺乏组比例最高,维生素D缺乏主要以维生素D_3缺乏为主且与骨密度及各功能性评分密切相关。 OBJECTIVE: To detect vitamin D level in perimenopausal women in Boluo area of ​​Huizhou by using tandem mass spectrometry, and to explore its correlation with bone mineral density. Methods: A total of 530 perimenopausal women aged 46-54 years at Boluo People’s Hospital from January 2013 to January 2016 were randomly selected as the research object, and further analyzed according to the composition of vitamin D. The main differences between the groups were observed Exist in vitamin D_2 or D_3, and finally use dual-energy X-ray examination of bone mineral density in each group to analyze whether there is a correlation with vitamin D levels. Results: The results showed that there were no significant differences among the four groups (P> 0.05). There were significant differences in the levels of vitamin D 3 between the two groups (P <0.05). The bone mineral density of lumbar spine and total hip in four groups showed an increasing trend with the increase of vitamin D levels, the difference was statistically significant (P <0.05). Conclusions: Serum vitamin D levels in perimenopausal women in Boluo, Huizhou based on tandem mass spectrometry were mostly in adequate state with the highest proportion of vitamin D deficiency group. The main deficiency of vitamin D deficiency was vitamin D 3 deficiency, Functional scores are closely related.