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目的了解沙苑子黄酮与维生素C对氢醌(HQ)致DNA损伤的拮抗作用,探讨HQ遗传毒作用的机制。方法分别用不同浓度沙苑子黄酮、维生素C与HQ共同作用于V79细胞后,用单细胞凝胶电泳技术(SCGE)检测DNA损伤的变化情况。结果SCGE结果显示,20、200 mmol/L维生素C和100 mg/L沙苑子黄酮对0.135、0.405 mmol/L浓度的HQ均有保护作用,表现为彗星细胞拖尾率、尾长、Olive尾矩降低,与对照组比较,差异有统计学意义(P<0.05)。而2000 mmol/L维生素C则加重了HQ的DNA损伤。结论在体外培养条件下,中、低浓度维生素C和中浓度沙苑子黄酮能拮抗HQ引起的DNA损伤。
Objective To investigate the antagonistic effect of Fructus Schisandrae Chinensis and Vitamin C on hydroquinone (HQ) -induced DNA damage and to explore the mechanism of genetic toxicity of HQ. Methods Different concentrations of Asarum flavonoids, vitamin C and HQ co-acting on V79 cells were detected by single cell gel electrophoresis (SCGE) DNA damage changes. Results The results of SCGE showed that 20,200 mmol / L vitamin C and 100 mg / L satsuma flavonoids could protect HQ at concentrations of 0.135 and 0.405 mmol / L, showing the tailing rate, tail length, Olive tail moment Decreased, compared with the control group, the difference was statistically significant (P <0.05). While 2000 mmol / L vitamin C aggravated DNA damage in HQ. Conclusion Under medium and low concentrations of vitamin C and medium concentration of Fructus Gardenia flavonoids can antagonize DNA damage caused by HQ.