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用人喉上皮细胞癌细胞系(HEP_2)的DNA转染人胚肺细胞(HEL),得到了5株基因转染细胞(GTC),命名为A5、B3、G8、D3和H3.这些GTC的染色体数在HEL的染色体数与两个亲代细胞(HEP-2和HEL)染色体的和数之间,感染人巨细胞病毒ADI69株后4d,D3比A5、B3、G8和H3有更多的荧光阳性细胞和更高的病毒滴度.在D3中HCMV复制随感染剂量的增大而增速.不同代数的D3对HCMV具有相似的敏感性,HCMV感染传至100代的D3,电镜证实仍可象原代D3复制大量的HCMV.永生性的D3可以用作分析调控HCMV复制的宿主细胞因子、分离培养HCMV等的工具.
Five human gene transfected cells (GTCs) were obtained by transfecting human embryonic lung cells (HEL) with DNA from human laryngeal epithelial carcinoma cell line (HEP_2) and named as A5, B3, G8, D3 and H3. These GTC chromosomes There was more fluorescence positive for D3 than A5, B3, G8 and H3 4 days after infection with human cytomegalovirus ADI69 strain between the chromosome number of HEL and the number of chromosomes of two parental cells (HEP-2 and HEL) Cells and higher viral titers.HCMV replication in D3 increased with the increase of infection dose.Different generations of D3 had similar sensitivity to HCMV, HCMV infection passed to 100 generations of D3, electron microscopy confirmed that still can be like Primary D3 replicates large amounts of HCMV, immortalized D3 can be used as a tool to analyze host cytokines that regulate HCMV replication, and to isolate and culture HCMV.