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目的:对金沙藤的化学成分及其抗氧化活性进行研究。方法:采用硅胶柱层析、凝胶层析等层析技术进行分离,通过NMR、MS等波谱技术确定化合物的结构,并采用TLC-生物自显影技术初步评价化合物的抗氧化活性。结果:分离并鉴定了10个化合物,分别为:槲皮素(Ⅰ)、紫云英苷(Ⅱ)、异槲皮苷(Ⅲ)、山萘酚-7-O-α-鼠李糖苷(Ⅳ)、芦丁(Ⅴ)、山萘酚-3-O-β-芸香糖苷(Ⅵ)、6-O-咖啡酸葡萄糖酯(Ⅶ)、α-葡萄糖(Ⅷ)、5-羟甲基糠醛(Ⅸ)及银杏醇(Ⅹ),从TLC-生物自显影色谱图显示,化合物Ⅰ、Ⅲ、Ⅴ、Ⅶ均显示了一定的抗氧化活性。结论:化合物Ⅰ、Ⅲ、Ⅴ、Ⅷ、Ⅸ、Ⅹ为首次从金沙藤中分离得到,综合得率及抗氧化活性考虑,异槲皮苷(Ⅲ)可用作金沙藤药材及其制剂质量标准研究用化学对照品。
OBJECTIVE: To study the chemical constituents and anti-oxidative activities of goldenrod. Methods: The compounds were isolated by silica gel column chromatography and gel chromatography. The structures of compounds were determined by NMR, MS and other spectroscopic techniques. The anti-oxidative activity of the compounds was also evaluated by TLC-autobiography. Results: Ten compounds were isolated and identified as quercetin (Ⅰ), astragalin (Ⅱ), isoquercetin (Ⅲ), kaempferol-7-O- (Ⅵ), 6-O-caffeic acid glucose ester (Ⅶ), α-glucose (Ⅷ), 5-hydroxymethylfurfural (Ⅸ) and ginkgol (Ⅹ). From TLC-BCD, the compounds Ⅰ, Ⅲ, Ⅴ and Ⅶ all showed certain antioxidant activity. Conclusion: The compounds Ⅰ, Ⅲ, Ⅴ, Ⅷ, Ⅸ and Ⅹ are isolated from Sanda for the first time. Considering the comprehensive yield and antioxidant activity, isoquercetrin (Ⅲ) can be used as the quality standard Research chemical reference substance.