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背景:脂质过氧化损害可导致学习记忆障碍,而漏芦能抑制脂质过氧化,具有提高细胞免疫功能、抗自由基过氧化等作用。目的:通过脑组织中过氧化脂质及脂褐质含量的变化,探讨漏芦乙醇提取物对D-半乳糖所致衰老小鼠学习记忆的影响。设计:随机对照实验。单位:沈阳药科大学药学院药理教研室,中国医科大学第一临床学院中医教研室,沈阳药科大学中药学院天然药化教研室。材料:实验于1994-02/10在沈阳药科大学药学院药理教研室完成。选取清洁级昆明种小鼠185只,随机分成7组:漏芦乙醇提取物3.125g/(kg·d)组30只、漏芦乙醇提取物1.56g/(kg·d)组30只、漏芦乙醇提取物0.78g/(kg·d)组30只、漏芦乙醇提取物0.39g/(kg·d)组15只、吡拉西坦组15只、空白对照组30只、模型组35只。方法:漏芦乙醇提取物3.125,1.56,0.78,0.39g/(kg·d)组分别灌服漏芦乙醇提取物3.125,1.56,0.78,0.39g/(kg·d),吡拉西坦组灌服吡拉西坦0.2g/(kg·d),空白对照组和模型组分别灌服同容积蒸馏水。除空白对照组外,其余各组小鼠每天均皮下注射50g/LD-半乳糖0.5mL/只,连续40d,建立小鼠衰老模型。40d后,采用跳台法及避暗法测定各组小鼠的学习记忆能力。主要观察指标:①各组小鼠在跳台法检测中的错误次数。②各组小鼠在避暗法检测中的错误次数及潜伏期的变化。③各组小鼠脑组织过氧化脂质及脂褐质含量生成的比较。结果:实验纳入小鼠185只,共169只进入结果分析。①各组小鼠在跳台法检测中的错误次数:与模型组比较,漏芦乙醇提取物3.125,1.56,0.78g/(kg·d)组均明显减少[(1.68±1.25),(0.92±0.73),(0.78±0.79),(0.97±0.67)次,P<0.01,P<0.001,P<0.01]。②各组小鼠在避暗法检测中的错误次数及潜伏期的变化:与模型组比较,漏芦乙醇提取物3.125,1.56,0.78g/(kg·d)组均明显减少(P<0.05或0.01);且潜伏期均有所延长(P<0.05或0.01)。③各组小鼠脑组织过氧化脂质及脂褐质含量生成的比较:与模型组比较,漏芦乙醇提取物3.125,1.56,0.78g/(kg·d)组均明显减少(P<0.05或0.01)。结论:漏芦乙醇提取物具有抗衰益智作用,其抗自由基过氧化、减少脑组织中过氧化脂质及脂褐质生成可能与其改善记忆障碍作用有关。
Background: Lipid peroxidation damage can lead to learning and memory disorders, while Leprudone can inhibit lipid peroxidation, and it has the functions of improving cellular immunity and anti-free radical peroxidation. OBJECTIVE: To investigate the effects of ethanol extract of Rhynchophylla on the learning and memory of aging mice induced by D-galactose through the changes of lipid peroxidation and lipofuscin content in brain tissue. Design: Randomized controlled trials. Unit: Department of Pharmacology, School of Pharmacy, Shenyang Pharmaceutical University; Department of Traditional Chinese Medicine, First Clinical College, China Medical University; Department of Natural Medicine, College of Traditional Chinese Medicine, Shenyang Pharmaceutical University. MATERIALS: The experiment was performed at the Department of Pharmacology, School of Pharmacy, Shenyang Pharmaceutical University from October to October 1994. 185 Kunming mice of clean grade were selected and randomly divided into 7 groups: 30 in the ethanol extract of 3.15g/(kg·d), and 30 in the ethanol extract of 1.56g/(kg·d). There were 30 ethanol extracts from the eucalyptus 0.78 g/(kg·d) group, 15 ethanol extracts from the lucifera 0.39 g/(kg·d) group, 15 piracetam groups, 30 blank control groups, and 35 model groups. only. Methods: Ethanol extracts of 3.12, 1.56, 0.78, and 0.39 g/(kg·d) were used to irrigated the ethanol extracts of Lekhorn 3.15, 1.56, 0.78, 0.39g/(kg·d) respectively. Piracetam group Impregnated piracetam 0.2g/(kg·d), the blank control group and the model group were fed the same volume of distilled water. Except for the blank control group, mice in the other groups were subcutaneously injected with 50 g/LD-galactose 0.5 mL/dose for 40 days consecutively to establish a mouse aging model. After 40 days, the ability of learning and memory of mice in each group was determined by step-down method and avoidance method. MAIN OUTCOME MEASURES: 1 The number of errors in each group of mice during the test of jumping platform. 2 The number of errors and the incubation period of the mice in the dark-detection method. 3Comparison of lipid peroxidation and lipofuscin content in brain tissue of each group. RESULTS: A total of 185 mice were included in the experiment and a total of 169 were involved in the result analysis. 1 The number of errors in each group of mice in the skip detection method: Compared with the model group, the ethanol extracts from the grape saplings (3.125, 1.56, 0.78g/(kg·d)) were significantly reduced [(1.68±1.25), (0.92±) 0.73), (0.78±0.79), (0.97±0.67) times, P<0.01, P<0.001, P<0.01]. 2Incorrect number of errors and incubation period in each group of mice in the evasion test:Compared with the model group, the ethanol extracts of schisandrae lucidum were significantly reduced in the 3.125, 1.56, and 0.78g/(kg·d) groups (P<0.05 or 0.01); and the incubation period was prolonged (P<0.05 or 0.01). 3Comparison of lipid peroxidation and lipofuscin content in brain tissue of each group:Compared with the model group, the concentrations of ethanol extracts from Alisma lucidum (3.125,1.56,0.78g/(kg·d)) were significantly decreased (P<0.05). Or 0.01). Conclusion: The ethanol extract of Phyllanthus has anti-aging and anti-aging effects. Its anti-free radical peroxidation, reduction of lipid peroxidation and lipofuscin production in brain tissue may be related to the improvement of memory impairment.