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从山东崅屿采集的黄棕壤中分离得到一株具有抗Mn(Ⅱ)和Mn(Ⅱ)氧化双重活性的芽胞杆菌,其最高Mn(Ⅱ)耐受浓度达到130mmol/L,对Mn(Ⅱ)的氧化活性为3.3μmol/(L·d)。通过个体形态与培养特征观测、生理生化反应、G+Cmol%测定和16SrDNA序列比对分析等鉴定,确定该菌株为巨大芽胞杆菌(Bacillus megaterium),命名为MB283。该菌株在添加Mn(Ⅱ)(10mmol/L)条件下比不添加Mn(Ⅱ)表现出相对较快的生长速率。采用高温培养并结合0.01%SDS处理,从MB283菌株筛选到一株发生内生质粒消除的突变株MB287,具有与野生菌株类似的锰耐受活性,且对Mn(Ⅱ)的氧化活性与野生菌株相比无明显改变,表明野生菌株MB283中与锰抗性和锰氧化相关的基因可能是定位于该菌的染色体上。
A Bacillus strain with double activity of Mn (Ⅱ) and Mn (Ⅱ) oxidation was isolated from yellow brown soil collected from Lanyu Island, Shandong Province. The maximum tolerated concentration of Mn (Ⅱ) was 130mmol / L, ) Had an oxidation activity of 3.3 μmol / (L · d). The strain was identified as Bacillus megaterium by the morphological and cultural characteristics observation, physiological and biochemical reactions, G + C mol% determination and 16S rDNA sequence alignment analysis. The strain showed a relatively fast growth rate compared with no addition of Mn (II) under the condition of adding Mn (II) (10 mmol / L). The strain MB287 was screened from MB283 strain with endogenous plasmid elimination by high temperature culture combined with 0.01% SDS. Manganese-tolerant activity was similar to that of wild strain MB287. The activity of Mn (Ⅱ) Compared to no significant change, indicating that the strain of wild-type MB283 and manganese resistance and manganese oxidation-related genes may be located on the chromosome of the bacteria.