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高温逆境下,植物膜透性的增加导致细胞代谢紊乱并诱导热激基因表达。脂肪酸是构成生物膜的主要物质,饱和脂肪酸的含量及饱和程度高,有利于保持膜在高温时的流动性和稳定性。本研究利用小麦(Triticum aestivum)耐热基因型TAM107和热敏感基因型中国春(CS)为对象,研究了高温胁迫对膜透性、叶绿素荧光参数Fv/Fm、膜脂组分及其相关脂肪酸去饱和酶基因(FAD7)表达的影响,结果表明,高温胁迫下,两基因型膜透性均增加,膜脂中的三烯脂肪酸含量下降,但TAM107变化幅度明显高于中国春,在脂肪酸水平上说明耐热小麦品种的膜系统较热敏感品种对高温逆境有较强的耐受性;中国春比TAM107中FAD7的表达对高温更加敏感,这直接影响了热胁迫前后膜脂中三烯脂肪酸含量的变化,在转录水平上说明了耐热小麦基因型较热敏感基因型对高温逆境有较强的耐受性。三烯脂肪酸含量在热胁迫前后的变化可作为一种新的耐热鉴定指标。
Under high temperature stress, the increase of plant membrane permeability leads to disorder of cell metabolism and induction of heat shock gene expression. Fatty acids constitute the main material of the biofilm, the content of saturated fatty acid and the degree of saturation are high, which is beneficial to maintain the fluidity and stability of the membrane at high temperature. In this study, the heat-tolerant genotype TAM107 of wheat (Triticum aestivum) and Chinese spring (CS) of heat-sensitive genotype were used to study the effect of high temperature stress on membrane permeability, chlorophyll fluorescence parameters Fv / Fm, lipid components and their related fatty acids Desaturase gene (FAD7) expression results showed that under high temperature stress, the membrane permeability of both genotypes increased, the content of triene fatty acids in membrane lipid decreased, but the amplitude of TAM107 was significantly higher than that of Chinese spring, at the fatty acid level Which indicated that the heat-sensitive wheat varieties were more tolerant to high temperature and stress than the heat-sensitive varieties. The expression of FAD7 in Chinese spring than TAM107 was more sensitive to high temperature, which directly affected the content of triene fatty acid Content changes, indicating that the heat-tolerant wheat genotypes at the transcriptional level more tolerant to high temperature stress than the heat-sensitive genotypes. The change of fatty acid content of triene before and after heat stress can be used as a new heat-resistant identification index.