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在室内条件下,采用菌丝生长速率法测定了几丁寡糖对烟草黑胫病菌的抑制作用,继而在温室盆栽和人工接种条件下,分别测定几丁寡糖、木霉、几丁质、几丁寡糖+木霉、几丁寡糖+木霉+几丁质等5种处理对烟草黑胫病的防治效果。结果表明,几丁寡糖在离体条件下对烟草黑胫病菌菌丝生长无抑制作用,但在盆栽试验中对烟草黑胫病具有显著防治效果。5种处理中,几丁寡糖单独处理对烟草黑胫病的防治效果最高,为57.81%,其次为几丁寡糖+木霉+几丁质和几丁寡糖+木霉处理,分别为53.49%和52.49%。在温室条件下对烟草植株施用几丁寡糖及人工接种黑胫病菌后10天内,几丁寡糖能显著提高烟草体内的SOD、POD、PPO和几丁质酶活性,且活性峰值分别比对照提高96.30%、136.36%、10.34%和9.10%。
Under indoor conditions, the mycelial growth rate method was used to determine the inhibitory effect of chitin oligosaccharides on tobacco blackleg. The chitooligosaccharides, Trichoderma, chitin, Chitin oligosaccharides + Trichoderma, chitin oligosaccharides + Trichoderma + chitin 5 kinds of treatment of tobacco black shank disease prevention and treatment. The results showed that chitooligosaccharides had no inhibitory effect on mycelial growth of Phytophthora nicotianae in vitro, but had significant control effect on tobacco black shank in pot experiment. Among the five treatments, the treatment with chitooligosaccharides alone had the highest control effect on tobacco black shank by 57.81%, followed by chitooligosaccharides + Trichoderma + chitin and chitin oligosaccharides + Trichoderma, respectively 53.49% and 52.49%. Chitin oligosaccharides could significantly increase the activities of SOD, POD, PPO and chitinase in tobacco leaves after applying chitin oligosaccharides and artificial inoculation with black shank in greenhouse under greenhouse conditions, and the activity peaks were significantly higher than those of control Increase 96.30%, 136.36%, 10.34% and 9.10%.