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目的 :观察白细胞介素 6 (interleukin 6 ,IL 6 )对血管升压素 (argipressin ,AVP)诱导的心脏成纤维细胞 (CFs)增殖及p2 7蛋白表达的影响。方法 :以培养的新生Sprague Dawley(SD)大鼠CFs为实验模型 ,四氮唑盐 (MTT)比色法检测细胞增殖 ,流式细胞分析仪 (FCM)技术测定细胞周期及p2 7蛋白表达阳性率。结果 :① 10 0 0 0U/mlIL 6作用于CFs 4 8h ,MTT法A值 (0 .38± 0 .0 1)显著高于基础状态组 (0 .32± 0 .0 1,P <0 .0 1) ;10 -7mol/LAVP和 10 0 0 0U/mlIL 6共同作用组CFs的A值 (0 .4 1± 0 .0 1)也明显高于AVP单独作用组 (0 .39± 0 .0 1,P <0 .0 1)。②IL 6作用组CFs的S期细胞百分率 (13.0 %± 3.5 % )和细胞增殖指数 (PI) (2 9.4 %± 1.9% )均高于基础状态组 (7.5 %± 1.0 %和 2 6 .0 %± 1.0 % ,P <0 .0 1) ,G0 /G1期细胞百分率 (70 .6 %± 1.9% )低于基础状态组 (74 .0 %± 1.0 % ) ;AVP +IL 6组CFs的S期细胞百分率和PI分别为 18.8%± 1.5 %和 35 .2 %± 1.6 % ,明显高于AVP组 (15 .5 %± 1.4 %和 31.4 %± 1.5 % ,P<0 .0 1) ,而G0 /G1期细胞百分率 (6 4 .8%± 1.6 % )明显低于AVP组 (6 8.6 %± 1.5 % ,P <0 .0 1)。 (3)IL 6组CFsp2 7蛋白表达阳性率 (72 .6 %± 2 .5 % )明显低于基础状态组 (78.4 %±
Objective: To observe the effect of interleukin 6 (IL 6) on the proliferation of cardiac fibroblasts (CFs) and the expression of p27 protein induced by argipressin (AVP). Methods: CFs from neonatal Sprague Dawley (SD) rats were used as experimental models. Cell proliferation was detected by MTT colorimetric assay. Cell cycle and p27 protein expression were determined by flow cytometry (FCM) rate. Results: (1) The effect of 10 000 U / ml IL 6 on CFs for 48 h was significantly higher than that of basal group (0.32 ± 0.01, P <0. 0 1). The A value (0.41 ± 0.01) of CFs treated with 10 -7 mol / L AVP and 10 000 U / ml IL 6 was also significantly higher than that of AVP alone group (0.39 ± 0. 0 1, P <0 .0 1). ② The percentage of S phase cells (13.0% ± 3.5%) and cell proliferation index (2 9.4% ± 1.9%) in CFs treated with IL 6 were significantly higher than those in basal cells (7.5% ± 1.0% and 26.0% ± 1.0%, P <0.01). The percentage of cells in G0 / G1 phase (70.6 ± 1.9%) was lower than that in basal group (74.0% ± 1.0% The percentage of staging cells and PI were 18.8% ± 1.5% and 35.2% ± 1.6%, respectively, which were significantly higher than those in AVP group (15.5% ± 1.4% and 31.4% ± 1.5%, P <0.01) The percentage of cells in G0 / G1 phase (64.4% ± 1.6%) was significantly lower than that in AVP group (8.6% ± 1.5%, P <0.01). (3) The positive rate of CFsp2 7 protein in IL 6 group (72.6% ± 2.5%) was significantly lower than that in basal group (78.4% ±