论文部分内容阅读
目的探讨改良手工操作免疫组织化学方法在病理诊断中的应用效果。方法应用即用型快捷免疫组化Max Vision TM试剂盒检测方法并加以适当改良,对常见肿瘤的常用抗体进行标记检测。结果抗原定位准确,背景清晰。假阳性、假阴性低,非特异性染色和掉片少。结论改良手工免疫组化染色效果良好,能满足常规病理诊断的需要,可以在中小型基层医院病理科推广应用,亦可用于样本量小的科研工作。
Objective To explore the application of improved manual operation immunohistochemistry in pathological diagnosis. Methods The rapid and rapid immunohistochemistry Max Vision TM kit detection method was applied and appropriately modified to detect the common anti-tumor antibodies. The results of antigen positioning accuracy, clear background. False positive, false negative low, non-specific staining and less pieces. Conclusion The improved immunohistochemical staining is effective and can meet the needs of routine pathological diagnosis. It can be applied in the pathology of small and medium-sized primary hospitals, and can also be used in scientific research with a small sample size.