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从枸杞岛海藻场和对照点贻贝场分别采取表层及底层水样,利用细菌通用引物,提取样品总DNA进行聚合酶链式反应(PCR)扩增,采用16SrDNA文库技术,对其细菌群落进行鉴定和分析。结果表明,海藻场表层和底层细菌群落分为5和6个门,贻贝场表层和底层细菌群落分为3和4个门,海藻场中细菌分类门数多于贻贝场内,优势菌均为变形细菌门,分别占克隆文库的比例为40.7%、50%、59.1%和48.1%。γ-变形菌纲为变形细菌门的优势亚群,各个文库中均含有许多未确定其分类的序列,即含有许多未被认知的微生物类群。变形菌门、厚壁菌门和放线菌门为所有水样共有,但在水层垂直分布及不同地理位置间存在较大差异。不同功能类群可能与其特殊生境密切相关。
The surface and bottom water samples were taken from the seaweed field of Goji Island and the control mussel field respectively. Total bacterial DNA was extracted by polymerase chain reaction (PCR) using universal bacterial primers. The 16S rDNA library was used to analyze the bacterial community Identification and analysis. The results showed that the bacterial communities in the surface and bottom of seaweed farm were divided into five and six gates. The bacterial communities in the surface and bottom of the mussel farm were divided into three and four gates. The number of bacteria in the algae farm was more than that in the mussel farm. All of them were deformed bacteria door, accounting for 40.7%, 50%, 59.1% and 48.1% respectively. γ-Proteobacteria are the dominant subgroups of the genera of the genera, each containing many unidentified sequences, ie, many unidentified microorganisms. Proteus, Firmicutes and actinomycetes for all water samples are common, but there is a big difference in the vertical distribution of water layer and different geographical locations. Different functional groups may be closely related to their special habitats.