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水稻类病变突变体chl1具有抗病性增强的类病变表型,对水稻白叶枯病和稻瘟病都具有很强的抗性。利用蛋白质组学技术分析chl1与其野生型之间的差异表达蛋白,探讨chl1类病变表型的形成和抗病反应的分子机制。利用荧光双向差异凝胶电泳(two-dimensional fluorescence difference gel electrophoresis,2D-DIGE)技术和质谱分析,chl1中共鉴定到70个差异表达的蛋白点,包括46个上调蛋白点和24个下调蛋白点。这些蛋白点参与不同的生物过程,包括防御相关、光合作用、氧化还原、氨基酸/蛋白质代谢、分子伴侣、碳水化合物代谢。对这些差异表达蛋白进行生物信息分析,推测它们所在的复杂调控网络可能参与chl1叶片细胞程序性死亡(programmed cell death,PCD)及其抗病性的调控。
Rice disease mutant chl1 has enhanced disease resistance phenotype and has strong resistance to bacterial blight and rice blast. Proteomics was used to analyze the differentially expressed protein between chl1 and its wild type to explore the molecular mechanism of chl1-like phenotype formation and disease resistance. A total of 70 differentially expressed protein spots, including 46 up-regulated protein spots and 24 down-regulated protein spots, were identified in chl1 using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and mass spectrometry. These protein spots are involved in different biological processes including defense-related, photosynthesis, redox, amino acid / protein metabolism, chaperones, and carbohydrate metabolism. Bioinformatics analysis of these differentially expressed proteins suggested that their complex regulatory networks may be involved in the programmed cell death (PCD) of chl1 and its regulation of disease resistance.