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目的:在前期研究基础上,深入研究蓝萼香茶菜的化学成分及其抗补体活性。方法:通过溶血试验,对蓝萼香茶菜各部位进行抗补体活性测试,以抗补体活性作为导向分离手段,运用多种色谱方法分离纯化蓝萼香茶菜中的化学成分,采用现代谱学技术进行结构鉴定,确定抗补体活性成分。结果:蓝萼香茶菜的正丁醇部位活性较强,从蓝萼香茶菜中分离得到11个化合物,分别为豆甾醇(1),豆甾醇-9(11)-烯-3-醇(2),蓝萼丁素(3),尾叶香茶菜丙素(4),山楂酸(5),科罗索酸(6),minheryinsⅠ(7),香叶木素(8),咖啡酸乙烯酯(9),咖啡酸(10),牡荆苷(11)。抗补体活性实验表明,化合物9,10以及前期分离得到的异槲皮苷、芦丁、槲皮素、槲皮素-3-甲醚、木犀草素、木犀草素-7-甲醚和芹菜素对经典途径的补体激活具有不同程度的抑制作用。结论:化合物2,4,6～9,11为首次从该植物中获得,咖啡酸的抗补体活性最强,CH50为0.041 g·L-1。
Objective: Based on previous studies, the chemical constituents and anti-complement activities of Chaxiang kohlrabi were studied in depth. Methods: The hemolysis test was carried out to test the anti-complement activity of various parts of Cymbopogon japonicus. Anti-complement activity was taken as the guide separation method. The chemical constituents of Cyperus orientalis were separated and purified by a variety of chromatographic methods. Technical identification of the structure to determine anti-complement active ingredients. Results: The activity of n-butanol was stronger in Camellia oleifera. Eleven compounds were isolated from blue-green calyx tea, which were stigmasterol, stigmasterol-9 (11) (4), maslinic acid (5), corosolic acid (6), minheryins Ⅰ (7), geraniol (8), coffee Vinyl acetate (9), caffeic acid (10), vitexin (11). Anti-complement activity test showed that compounds 9, 10 and isoquercitin, rutin, quercetin, quercetin-3-methyl ether, luteolin, luteolin-7-methyl ether and celery Supression of the classical pathway of complement activation has varying degrees of inhibition. CONCLUSION: Compounds 2,4,6 ~ 9,11 were obtained from this plant for the first time. Caffeic acid had the highest anti-complement activity with a CH50 of 0.041 g · L-1.