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目的探讨布地奈德对哮喘气道重塑大鼠尾加压素-Ⅱ(U-Ⅱ)及其mRNA表达的影响。方法 24只清洁级雄性SD大鼠分为3组:对照组、哮喘组和布地奈德组。以卵清白蛋白(OVA)致敏激发法制备哮喘大鼠气道重塑模型,应用图像分析技术测定支气管壁厚度(Wat)和平滑肌厚度(Wam),酶联免疫吸附法(ELISA)测定血清和支气管肺泡灌洗液(BALF)中U-Ⅱ的浓度,免疫组化法和逆转录-聚合酶链反应(RT-PCR)法分别检测U-Ⅱ蛋白和U-ⅡmRNA在肺组织中的表达。结果①哮喘组Wat和Wam均显著高于对照组,布地奈德组Wat和Wam均显著低于哮喘组(均P<0.01);②哮喘组血清和BALF中U-Ⅱ的浓度均显著高于对照组,布地奈德组上述指标均显著低于哮喘组(均P<0.01);③免疫组化及RT-PCR结果显示,肺组织中U-Ⅱ蛋白及其mRNA的表达,哮喘组显著高于对照组,布地奈德组较哮喘组显著减弱(均P<0.01);④相关性分析显示,大鼠肺组织Wat与U-Ⅱ蛋白、U-ⅡmRNA表达呈正相关(均P<0.01),Wam与U-Ⅱ蛋白、U-ⅡmRNA表达呈正相关(均P<0.01)。结论布地奈德对哮喘气道重塑的拮抗作用,可能通过抑制U-Ⅱ的表达起作用。
Objective To investigate the effect of budesonide on urotensin-Ⅱ (U-Ⅱ) and its mRNA expression in airway remodeling rats with asthma. Methods Twenty-four male SD rats were divided into three groups: control group, asthma group and budesonide group. The airway remodeling model of asthmatic rats was established by the sensitization method of ovalbumin (OVA). The thickness of the bronchial wall (Wat) and the thickness of the smooth muscle (Wam) were measured by image analysis. The serum and The concentration of U-Ⅱ in BALF, the expression of U-Ⅱprotein and U-ⅡmRNA in lung tissue were detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) Results ① Wat and Wam in asthma group were significantly higher than those in control group, and Wat and Wam in budesonide group were significantly lower than those in asthma group (all P <0.01). ② The concentrations of U-Ⅱ in serum and BALF in asthma group were significantly higher than those in control group In the control group and budesonide group, the above indexes were significantly lower than those in the asthma group (all P <0.01); ③The expression of U-Ⅱprotein and its mRNA in the lung tissue was significantly higher than that in the asthma group by immunohistochemistry and RT-PCR (P <0.01) .④The correlation analysis showed that there was a positive correlation between Wat and U-Ⅱprotein expression and U-ⅡmRNA expression (all P <0.01) in the control group and budesonide group compared with asthma group There was a positive correlation between Wam and U-Ⅱ protein and U-ⅡmRNA (all P <0.01). Conclusion Budesonide antagonizes the airway remodeling of asthma and may play an important role by inhibiting the expression of U-Ⅱ.