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单层贴壁的培养方法能够在无血清的情况下将胚胎干细胞(ES细胞)诱导分化形成神经样的细胞.重编程小鼠脑膜细胞产生的多能干细胞(iPS细胞)C5,也可以运用诱导ES细胞神经发生的方法来诱导神经特异性标志基因Sox1,Sox3,Pax6,Nestin和Tuj1的表达;与之相反,随着分化的进行,ES细胞特异性的标志基因Oct4和Nanog的表达量迅速降低.通过细胞免疫荧光技术,可以检测到大量Pax6和Nestin阳性的神经前体细胞的存在,并且随着时间的推移,这些前体细胞能够分化形成3类中枢神经系统的细胞,分别是神经元、星形胶质细胞和少突胶质细胞.体外诱导iPS细胞形成的个体特异性细胞可以作为研究遗传类疾病机制的工具,并且可用来治疗机能紊乱和年老的神经组织.此外,脑膜细胞由于高表达胚性调控因子Sox2,更容易被逆分化形成iPS细胞,为此将更加胜任于临床治疗应用.
Monolayer adherent culture can differentiate embryonic stem cells (ES cells) into neuron-like cells in the absence of serum, and pluripotent stem cells (iPS cells) C5 produced by reprogramming mouse meningocytes can also be induced using induction ES cell neurogenesis to induce the expression of the neuron-specific marker genes Sox1, Sox3, Pax6, Nestin, and Tuj1; in contrast, the expression of ES cell-specific marker genes Oct4 and Nanog decreased rapidly as differentiation progressed By immunofluorescence, a large number of Pax6 and Nestin positive neuronal progenitor cells can be detected and, over time, these precursor cells can differentiate into cells of three types of central nervous system, namely neurons, Astrocytes and oligodendrocytes.Individual-specific cells induced in vitro to induce iPS cells can be used as a tool to study the mechanisms of genetic diseases and can be used to treat disorders and aged nerve tissue.In addition, High expression of embryonic regulatory factor Sox2, more easily reverse degeneration to form iPS cells, for which will be more competent in clinical treatment applications.