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目的:研究红景天苷(salidroside)对慢性重复悬尾应激模型(repeated tail-suspension test,R-TST)小鼠的保护作用。方法:昆明种小鼠50只,随机分为5组,即对照组、应激组、红景天苷高(60 mg.kg-1)、中(30 mg.kg-1)、低剂量组(15 mg.kg-1)。采用重复悬尾应激模型,重复悬吊应激11 d,每天应激1次,每次2 h,并每隔3 d增加1 h,每次应激前30 min ig给予相应剂量的红景天苷或生理盐水,并于末次应激结束30 min后眼眶取血,用RIA测定血清中睾酮含量。摘取脾脏、胸腺、肾上腺和睾丸,称重并计算脏器指数。取肾上腺和睾丸用4%多聚甲醛固定并制作组织切片,HE染色,显微镜下观察形态学变化。结果:应激后小鼠体重增长程度明显受抑,质量增加幅度仅为对照组的27%;肾上腺指数上升,为对照组的164%;脾脏指数下降为对照组的70%;肾上腺皮质肥厚,髓质萎缩甚至消失。应激组小鼠血浆睾酮水平显著低于对照组(P<0.01)。应激前30min给予低剂量红景天苷可逆转应激导致的体重增加减缓,降低肾上腺指数,脾脏指数增加;肾上腺皮质肥厚及髓质萎缩等病理症状减轻,睾酮恢复至正常水平。但中剂量组和高剂量红景天苷组上述作用不显著。结论:低剂量红景天苷可显著改善应激小鼠体重增加减缓、肾上腺指数升高、脾脏指数下降、肾上腺皮质肥厚、髓质萎缩和结构层次模糊等,且能显著抑制重复悬尾应激所引起的睾酮水平低下并使其恢复正常,提示红景天苷可能通过改善应激所致的睾酮水平降低及维持肾上腺的正常结构和正常生理功能发挥抗应激作用。
Objective: To study the protective effect of salidroside on mice with repeated tail-suspension test (R-TST). Methods: Fifty Kunming mice were randomly divided into 5 groups: control group, stress group, salidroside high (60 mg.kg-1), medium (30 mg.kg-1) (15 mg.kg-1). Repeated suspension stress model was used to suspend the stress for 11 days. The stress was repeated once a day for 2 hours and every 1 day for 1 hour. The corresponding dose of rhodiola Day glycosides or saline, and in the last 30 min after the end of the eye orbital blood, using RIA determination of serum testosterone levels. Spleen, thymus, adrenal, and testis were harvested, weighed, and organ indices calculated. Adrenal gland and testis were fixed with 4% paraformaldehyde and made tissue sections, HE staining, microscopic observation of morphological changes. Results: The body weight growth of mice was significantly inhibited after stress, with the increase of mass being only 27% of the control group; the adrenal index increased to 164% of the control group; the spleen index dropped to 70% of the control group; adrenocortical hypertrophy, Medulla atrophy or even disappear. The level of plasma testosterone in stress group was significantly lower than that in control group (P <0.01). Low dose of salidroside 30min before stress can reverse the stress-induced weight gain slowed down, reducing the adrenal index, spleen index increased; adrenal hypertrophy and medullary atrophy and other pathological symptoms, testosterone returned to normal levels. But the middle dose group and high dose salidroside group the above effect is not significant. Conclusions: Salidroside at low dose can significantly reduce the weight gain, the adrenal index, the spleen index, adrenocortical hypertrophy, medullary atrophy and structural disorder in stress mice, and can significantly reduce the repetitive tail stress The testosterone levels are low and normalized, suggesting that salidroside may exert anti-stress effects by reducing the level of testosterone induced by stress and maintaining the normal structure and normal physiological function of the adrenal glands.