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目的探索microRNA在四氯化碳(CCl4)诱导的大鼠纤维化肝组织中的差异表达及其意义。方法采用microRNA高通量测序分析技术检测对照组(n=10)与模型组(n=10)肝脏组织microRNA表达,对差异microRNA进行靶基因预测,对靶基因进行基因本(GO)分析、pathway分析。结果对照组与模型组间筛选出37个差异microRNA,上调29个,下调8个;从microRNA-基因网络图中发现关键上调为miR-184、miR-10b-5p、miR-199a-3p等;关键下调为miR-200b-3p、miR-199a-5p、miR-125b-5p等。结论模型组microRNA表达谱变化明显,肝纤维化的形成与microRNA调控细胞增殖、凋亡、细胞周期等有关。
Objective To explore the differential expression of microRNA in rat liver fibrosis induced by carbon tetrachloride (CCl4) and its significance. Methods The microRNA expression in the liver of the control group (n = 10) and the model group (n = 10) was detected by microRNA high-throughput sequencing analysis, the target genes were predicted by differential microRNA, the target gene was analyzed by GO, analysis. RESULTS: Thirty-seven differentially expressed microRNAs were screened from control group and model group, 29 of them were up-regulated and down-regulated by 8. From the microRNA-gene network map, miR-184, miR-10b- The key down-regulation is miR-200b-3p, miR-199a-5p, miR-125b-5p and so on. Conclusion The expression of microRNA in the model group changes obviously. The formation of hepatic fibrosis is related to the regulation of proliferation, apoptosis and cell cycle by microRNA.