在组织培养中常用高压蒸气灭菌法对一些物品及试剂进行灭菌,但是由于使用方法等问题,经常出现因灭菌不彻底而导致组织培养污染的现象。如何正确使用高压蒸气灭菌方法,我们进行了比较研究,现报告如下:1　材料与方法1.1　材料　标准菌株大肠杆菌及金黄色葡萄球菌(大医附二院... In tissue culture commonly used high-pressure steam sterilization of some articles and reagents sterilization, but due to the use of methods and other issues, often due to sterilization is not complete and lead to tissue culture pollution phenomenon. How to properly use high-pressure steam sterilization method, we conducted a comparative study, are as follows: 1 Materials and methods 1.1 Materials Strain E. coli and Staphylococcus aureus (large medical attached to the second hospital ...