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为了解青岛地区丙型肝炎病毒(HCV)基因型分布情况.采用逆转录一套式聚合酶链反应和限制性片段长度的多态性分析方法,对154份HCV-RNA阳性血清进行了HCV-5’末端非编码区酶切分型.结果:HCVⅡ型占82.5%、HCVⅢ型占16.9%、Ⅱ/Ⅲ混合型占0.6%;在各临床类型中,HCV型的感染率随着肝病的进展而逐渐增加.提示:HCVⅡ型为青岛地区HCV感染的优势株,并且与肝病的进展密切相关.今后在丙肝的诊断、治疗及疫苗开发等研究方面,应将HCVⅡ型作为重点.
To understand the distribution of hepatitis C virus (HCV) genotypes in Qingdao, 154 HCV-RNA positive sera were analyzed by reverse transcription polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) 5 ’end non-coding region.Results: HCVⅡtype accounted for 82.5%, HCVⅢtype accounted for 16.9%, Ⅱ / Ⅲ mixed accounted for 0.6%; in each clinical type, HCV-type infection rate with the progress of liver disease , Suggesting that HCV Ⅱ is the predominant strain of HCV infection in Qingdao and is closely related to the progress of liver disease.In the future, HCV Ⅱ should be the focus of the study on the diagnosis, treatment and vaccine development of HCV.