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采用95%乙醇提取药材,提取液回收至无醇味后依次过聚酰胺树脂、MCI树脂、中压制备液相色谱和制备型高效液相色谱,从剑叶凤尾蕨全草中分离得到8个二萜类化合物和2个倍半萜类化合物。质谱(ESI-MS)数据和核磁共振(NMR)数据与文献数据对比鉴定分离得到的化合物分别为ent-3β-hydroxy-kaur-16-en-19-al(1),4-epi-kaurenic acid(2),mitrekaurenone(3),7β,16α,17-trihydroxy-ent-kauran-19-oic acid(4),crotonkinin E(5),crotonkinin F(6),pterisolic acid A(7),pterisolic acid C(8),(2R)-pterosin P(9)和dehydropterosin B(10)。化合物1~6首次从凤尾蕨属中分离得到,化合物7~10首次从剑叶凤尾蕨中获得。细胞毒活性测试表明化合物5~8具有一定的抑制人结肠癌细胞HCT-116、肝癌细胞Hep G2和人胃癌细胞BGC-823的活性。
The medicinal materials were extracted with 95% ethanol, the extract was recovered to non-alcoholic flavor, followed by polyamide resin, MCI resin, medium pressure preparative liquid chromatography and preparative high performance liquid chromatography. Diterpenoids and two sesquiterpenoids. The compounds obtained by ESI-MS and NMR data comparison with the literature data were respectively identified as ent-3β-hydroxy-kaur-16-en-19-al Crotonkinin E (5), crotonkinin F (6), pterisolic acid A (7), pterisolic acid (2), 7β, 16α, 17-trihydroxy-ent-kauran- C (8), (2R) -pterosin P (9) and dehydropterosin B (10). Compounds 1 ~ 6 were isolated from Pteridium spp. For the first time, and compounds 7 ~ 10 were obtained from Pteridophyta spp. For the first time. Cytotoxicity assay showed that compounds 5-8 had certain inhibitory effects on human colon cancer cells HCT-116, hepatoma Hep G2 and human gastric cancer BGC-823.