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目的 :研究运动诱导的大鼠心肌细胞HSP72mRNA表达与大鼠心肌细胞超微结构改变的可能关系。方法 :将 36只SD大鼠随机分为安静对照组 (C)、1周运动组 (T1)、2周运动组 (T2 )和3周运动组 (T3)。安静对照组不运动 ,T1组、T2组和T3组以 75 %VO2 max强度进行跑台运动 ,分别运动 1周、2周、3周 ,每周运动 6天 ,每天 1小时。在末次运动结束后 2 4小时以RT -PCR法检测大鼠心肌细胞热休克蛋白 72mRNA的表达 ;以常规电镜检测观察大鼠心肌细胞超微结构的变化。结果 :①T1和T2组HSP72mRNA表达明显多于C组和T3组。②以C组心肌细胞电镜表现为正常 ,则各组大鼠电镜表现的心肌损伤程度为T1组 >T2组 >T3组。结果提示 :运动不同时期造成心肌损伤程度不同的原因可能与运动后心肌细胞HSP72mRNA表达量不同有关。HSP72mRNA表达可能为运动后心肌保护的分子机理。
Objective: To investigate the possible relationship between exercise-induced HSP72 mRNA expression in rat cardiomyocytes and ultrastructural changes in rat cardiomyocytes. Methods: Thirty-six Sprague Dawley rats were randomly divided into three groups: control group (C), exercise group (T1), exercise group (T2) and exercise group (T3) The quiet control group did not exercise, T1 group, T2 group and T3 group treadmill exercise intensity of 75% VO2 max, exercise 1 week, 2 weeks, 3 weeks, exercise 6 days a week, 1 hour a day. The expression of heat shock protein 72 mRNA in rat cardiomyocytes was detected by RT-PCR 24 hours after the last exercise. The ultrastructural changes of rat myocardial cells were observed by conventional electron microscopy. Results ① The expression of HSP72 mRNA in T1 and T2 groups was significantly higher than that in C and T3 groups. (2) Electrocardiogram was normal in group C, and the degree of myocardial damage in group E was T1> T2> T3. The results suggest that the different causes of myocardial injury at different periods of exercise may be related to the different expression of HSP72mRNA in cardiomyocytes after exercise. HSP72mRNA expression may be the molecular mechanism of myocardial protection after exercise.