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目的:研究重组人生长激素(rhGH)联合氟尿嘧啶(5-FU)在体外对生长激素受体(GHR)不同表达状态人胃癌细胞株的影响。方法:选用GHR高表达的SGC-7901和GHR低表达的MKN-45胃癌细胞株。每种细胞株分为空白对照组、5-FU处理组、rhGH处理组和联合处理组。采用四基偶氮唑蓝(MTT)比色法和流式细胞术,分析不同浓度rhGH及其与5-FU合用,对人胃癌细胞株的生长抑制、凋亡、细胞周期和增殖指数(PI)等的影响。结果:SGC-7901细胞株:与空白对照组比,各浓度rhGH组的生长率、G2/M期比例和PI显著升高,细胞凋亡率显著降低(P<0.05);与5-FU处理组比,各浓度rhGH+5-FU组的G2/M期比例和PI显著升高,抑制率和细胞凋亡率显著降低(P<0.05)。MKN-45细胞株:不同浓度rhGH对该细胞株的分裂增殖无明显影响(P>0.05)。联合处理组与5-FU处理组间的细胞抑制率、凋亡率和PI也无显著性差异(P>0.05)。结论:rhGH在体外能促进高表达GHR的胃癌细胞增殖,减弱5-FU的抗癌作用,但对低表达GHR的胃癌细胞无明显影响,也不能明显干扰5-FU的抗癌作用。
Objective: To investigate the effect of recombinant human growth hormone (rhGH) combined with 5-fluorouracil (5-FU) on human gastric cancer cell lines with different expression of growth hormone receptor (GHR) in vitro. Methods: SGC-7901 with high GHR expression and MKN-45 gastric cancer cell line with low GHR expression were selected. Each cell line was divided into blank control group, 5-FU treatment group, rhGH treatment group and combined treatment group. MTT assay and flow cytometry were used to analyze the effects of different concentrations of rhGH and 5-FU on the growth, apoptosis, cell cycle and proliferation index (PI) of human gastric cancer cell lines ) And other effects. Results: Compared with the blank control group, the growth rate of SGC-7901 cells, the ratio of G2 / M phase and the PI of each concentration of rhGH group were significantly increased and the apoptosis rate of rhGH group was significantly decreased (P <0.05) The ratio of G2 / M phase and PI in each concentration of rhGH + 5-FU group were significantly increased, and the inhibition rate and apoptosis rate were significantly decreased (P <0.05). MKN-45 cell line: different concentrations of rhGH had no significant effect on the cell division (P> 0.05). There was no significant difference between the combined treatment group and the 5-FU treatment group in cell inhibition rate, apoptosis rate and PI (P> 0.05). CONCLUSION: rhGH can promote the proliferation of gastric cancer cells with high expression of GHR in vitro and attenuate the anticancer effect of 5-FU. However, rhGH has no obvious effect on gastric cancer cells with low expression of GHR, and can not interfere with the anticancer effect of 5-FU.