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为了观察硫氧还蛋白（ＴｒｘＡ）促进外源蛋白在大肠杆菌中可溶性表达的作用，我们从质粒ｐＥＴ－３２ａ（＋）上克隆了ｔｒｘＡ基因，构建了ＴｒｘＡ表达质粒ｐＴ－ＴｒｘＡ。将该质粒与其它蛋白基因的表达质粒共同转化Ｅｃｏｌｉ并同时获得表达。结果表明，共表达ＴｒｘＡ可以明显促进外源蛋白，如甲状旁腺激素相关蛋白（ＰＴＨｒＰ）、ＰＴＨｒＰ受体（ＰＴＨｒＰ－Ｒ）和血管内皮生长因子（ＶＥＧＦ）的可溶性表达。说明共表达ＴｒｘＡ有助于外源蛋白的正确折叠，防止包涵体的形成，增加可溶性蛋白的含量
In order to observe the role of thioredoxin (TrxA) in promoting the soluble expression of foreign proteins in E. coli, we cloned the trxA gene from plasmid pET-32a (+) and constructed the TrxA expression plasmid pT-TrxA. The plasmid and other protein gene expression plasmid co-transformation E.coli and at the same time obtain the expression. The results showed that co-expression of TrxA could significantly promote the soluble expression of foreign proteins such as PTHrP, PTHrP-R and VEGF. Co-expression of TrxA contributes to the correct folding of foreign proteins, preventing the formation of inclusion bodies and increasing the content of soluble proteins