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目的和方法 :采用放射性同位素法观察发热和低体温大鼠下丘脑腺苷酸环化酶 (AC)及磷酸二脂酶(PDE)活性的经时变化。结果 :鲜酵母皮下注射后 4h引起体温明显升高 (P <0 0 1) ,并长时维持在高位水平 ,AC活性在造模后 3h达峰值 (P <0 0 5 ) ,其后活性下降并渐至正常 ,PDE活性在整个过程中略有上升 ;安痛定腹腔注射后15min动物即呈现低体温状态 (P <0 0 1) ,AC活性在 15min时有一短暂上扬后至 4 5min降到最低点 (P <0 0 5 ) ,之后活性逐步回升 ,而PDE活性则在正常水平上下小幅波动。结论 :下丘脑AC活性的增强可能是动物体温升高的重要因素 ,而PDE活性并未相应显著升高则又造成了体温长时的偏高 ;在低体温模型中 ,AC活性的明显下降表明cAMP可能与动物的低温状态有关。
PURPOSE AND METHODS: The changes of hypothalamic adenylyl cyclase (AC) and phosphodiesterase (PDE) activity in fever and hypothermia rats were observed by radioisotope method. Results: The body temperature increased significantly 4 h after hypodermic injection of fresh yeast (P <0.01), and remained at a high level for a long time. AC activity peaked at 3 h after modeling (P <0.05), and then decreased PDE activity increased slightly during the whole process. At 15 minutes after intraperitoneal injection of Antongding, animals showed hypothermia (P <0.01), and AC activity dropped to a minimum after 45 minutes after 15min (P <0.05), then the activity gradually rose, while the PDE activity fluctuated slightly from the normal level. CONCLUSIONS: The enhancement of AC activity in the hypothalamus may be an important factor for the increase of body temperature in animals. However, no significant increase in PDE activity leads to long-term high body temperature. In the hypothermia model, the obvious decrease in AC activity indicates cAMP may be related to the animal’s hypothermia.