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目的观察丙型肝炎病毒(HCV)F基因对c-myc启动子转录活性的影响,探讨HCV F基因表达产物参与肝细胞癌变的机制。方法构建c-myc启动子的萤光素酶报告质粒,与HCV-F和HCV-C的表达质粒共转染人肝癌细胞系HepG 2细胞,测定萤光素酶的活性,分析HCV-F、HCV-C对c-myc启动子转录活性的影响。结果在HepG 2细胞中,HCV-C对c-myc启动子的转录具有明显的促进作用,启动子的转录活性比对照组提高约7倍,且在HCV-C的剂量为2~10μg时,其作用具有剂量依赖性;HCV-F对c-myc启动子转录活性也具有激活作用,在剂量为2μg时,启动子的转录活性约为对照组的3倍,c-myc启动子对HCV-F的剂量依赖激活作用不明显。结论 HCV-F相比HCV-C不能有效的调节c-myc启动子转录活性,HCV-C起主要的调节功能。
Objective To investigate the effect of hepatitis C virus (HCV) F gene on the transcriptional activity of c-myc promoter and to explore the mechanism of HCV F gene expression in hepatocellular carcinogenesis. Methods The luciferase reporter plasmid of c-myc promoter was constructed and transfected into HepG 2 cells with HCV-F and HCV-C expression plasmids to determine luciferase activity. HCV-F, Effect of HCV-C on c-myc promoter transcriptional activity. Results In HepG 2 cells, HCV-C promoted the transcription of c-myc promoter, and the transcriptional activity of the promoter was about 7 times higher than that of the control group. When the dose of HCV-C was 2 ~ 10μg, The effect of HCV-F on the transcriptional activity of c-myc promoter was also dose-dependent. At the dose of 2μg, the transcriptional activity of the promoter was about 3 times that of the control group, D dose-dependent activation is not obvious. Conclusion HCV-F can not effectively regulate the transcriptional activity of c-myc promoter compared with HCV-C, and HCV-C plays a major regulatory role.