采用配对病例对照研究，探讨先心病与柯萨奇病毒B3、B4感染的关系，结果显示：病例组与对照组患儿CVB3、CVB4．IgG抗体阳性率分别为59．3％、54．3％和53．6％、45．7％，差异天显著性（P值均＞0．05）；经单因素和多因素回归分析均未发现CVB3、CVB4感染与先心病有病因学联系（P＞0．05） A matched case-control study was conducted to investigate the relationship between Coxsackievirus B3 and B4 infection in congenital heart disease. The results showed that CVB3 and CVB4 were positive in the case and control groups. The positive rates of IgG antibodies were 59.3%, 54.3% and 53.6%, 45.7%, respectively, with significant difference (P> 0.05). No single factor or multiple regression analysis CVB3 and CVB4 infection were found to be associated with etiology (P> 0.05)