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正交实验的方差和极差分析表明,胡杨雌花诱导的愈伤组织和芽的效果明显优于雄花;雌花诱导愈伤组织的最适培养基是B5添加1.0mg·L-16-BA和1.0mg·L-1NAA,诱导芽的最适培养基为MS+0.5mg·L-16-BA+0.2mg·L-1NAA,诱导根的最适培养基为1/2MS+0.5mg·L-1IBA。
The variance and range analysis of orthogonal experiment showed that the callus and bud induced by female flower of Populus euphratica were obviously better than male flower. The optimum culture medium of female flower induced callus was B5 adding 1.0 mg · L-16-BA and 1.0 The optimum medium for induction of shoots was MS + 0.5 mg · L-16-BA + 0.2 mg · L-1 NAA and the optimum medium for root induction was 1/2 MS + 0.5 mg · L -1 IBA .