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作者根据金黄色葡萄球菌Cowan株带有一种表面蛋白能与免疫球蛋白IgG的Fc部分结合的特性,在病毒感染的细胞悬液中加入特异性抗血清后,再加入金黄色葡萄球菌,则表面粘附细菌的细胞即为病毒感染的细胞。作者选用滤泡性口腔炎病毒(VSV)作为实验模型,病毒用地鼠肾细胞(BHK),在含有5%胎牛血清的Eagle′s培养液中进行悬浮培养,并经蔗糖密度梯度离心沉淀法纯化。特异性细菌粘附试验,是将VSV感染的BHK细胞用磷酸盐缓冲盐水(PBS)洗2次,再用1%戊二醛固定10分钟,并用PBS洗2次,然后用1∶20的
According to the Staphylococcus aureus Cowan strain with a surface protein that binds to the Fc portion of immunoglobulin IgG, specific antisera are added to the virus-infected cell suspension followed by Staphylococcus aureus, Bacterial cells are virus-infected cells. The authors selected follicular stomatitis virus (VSV) as an experimental model, and the virus was cultured in hamster kidney cells (BHK) in Eagle’s medium containing 5% fetal bovine serum and subjected to sucrose density gradient centrifugation purification. Specific bacterial adhesion assays were performed by shaking VSV-infected BHK cells twice with phosphate buffered saline (PBS), fixing with 1% glutaraldehyde for 10 minutes and washing twice with PBS, followed by a 1:20