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目的探讨血清葡萄糖-6-磷酸异构酶(GPI)水平与各种自身免疫性疾病(AID)相关性及其诊断价值。方法采用酶联免疫吸附试验(ELISA)检测各组AID患者包括类风湿性关节炎(RA)组、系统性红斑狼疮(SLE)组、干燥综合症(SS)组和健康体检者血清GPI浓度并进行比较。结果当GPI以0.28μg/ml为临界值时,RA组、SLE组、SS组、健康对照组阳性率分别为68.7%、17.6%、23.1%、2.4%,RA组的GPI阳性率显著高于其他AID组及健康对照组,与其他各组有显著性差别(P<0.01)。SLE组及SS组与健康对照组均有显著性差别(P<0.01)。GPI对RA诊断的灵敏度和特异性分别为68.7%、87.1%。结论血清GPI在各AID组中,以RA组升高最明显,对RA诊断具有较高的灵敏度和特异性,可作为RA诊断的新指标。血清GPI与RF联合检测可提高RA的诊断率。
Objective To investigate the relationship between serum glucose-6-phosphate isomerase (GPI) and various autoimmune diseases (AIDs) and its diagnostic value. Methods Serum GPI levels of AID patients including rheumatoid arthritis (RA) group, systemic lupus erythematosus (SLE) group, Sjogren’s syndrome (SS) group and healthy subjects were detected by enzyme linked immunosorbent assay (ELISA) Compare. Results The positive rates of GPI in RA group, SLE group, SS group and healthy control group were 68.7%, 17.6%, 23.1% and 2.4% respectively when the GPI was 0.28μg / ml. The positive rate of GPI in RA group was significantly higher than that in RA group Other AID group and healthy control group, with the other groups were significantly different (P <0.01). SLE group and SS group and the healthy control group were significantly different (P <0.01). The sensitivity and specificity of GPI for the diagnosis of RA were 68.7% and 87.1% respectively. Conclusions Serum GPI is the most significant factor in the diagnosis of RA with high sensitivity and specificity in RA patients. Serum GPI and RF combined detection can improve the diagnostic rate of RA.