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目的 研究热化疗对C6细胞凋亡的影响 ,评价热化疗抑制细胞增殖的价值。方法 将不同浓度的丝裂霉素直接作用于体外培养的C6胶质瘤细胞 ,接种后 2 0h、48h置于 42 .7℃恒温箱中加温 2次 ,每次 3 0min。观察丝裂霉素对C6细胞的抑制率、生长曲线的影响及热疗、化疗、热化疗对C6细胞凋亡的诱导作用。结果 丝裂霉素在 1.5 μg/ml浓度时杀伤作用达高峰 (P <0 .0 0 1) ,再加大浓度并不增加杀伤作用 (P >0 .0 5 ) ;透射电镜、FCM、TUNEL均观察到热疗、化疗、热化疗诱导细胞凋亡的作用 (P <0 .0 0 1) ;热化疗有协同作用 (P <0 .0 0 1)。结论 ①热疗、化疗、热化疗均抑制C6细胞增殖。②热疗、化疗、热化疗诱导细胞凋亡为其抗癌机制之一。③热疗能提高化疗药物的细胞毒性作用。
Objective To study the effect of thermochemotherapy on C6 cell apoptosis and evaluate the value of thermochemotherapy in inhibiting cell proliferation. Methods Different concentrations of mitomycin C were directly treated in C6 glioma cells cultured in vitro, after inoculation 20h, 48h placed in 42.7 ℃ incubator heated twice, each 30min. The inhibitory rate of mitomycin C on C6 cells and the growth curve were observed, and the induction of C6 cells apoptosis was induced by hyperthermia, chemotherapy and thermochemotherapy. Results Mitomycin at the concentration of 1.5 μg / ml had the highest cytotoxicity (P <0.01), but did not increase the cytotoxicity (P> 0.05) at the same concentration. Transmission electron microscopy, FCM, TUNEL The effects of hyperthermia, chemotherapy and thermotherapy on apoptosis were observed (P <0.01). The thermochemotherapy had a synergistic effect (P <0.01). Conclusion ① hyperthermia, chemotherapy and thermotherapy can inhibit the proliferation of C6 cells. ② hyperthermia, chemotherapy, thermotherapy induced apoptosis as one of its anti-cancer mechanism. ③ hyperthermia can improve the cytotoxic effect of chemotherapy drugs.